PMID: 8603925Mar 1, 1996Paper

The neurotrophin receptor, gp75, forms a complex with the receptor tyrosine kinase TrkA

The Journal of Cell Biology
A H RossD E Wolf

Abstract

The high-affinity NGF receptor is thought to be a complex of two receptors , gp75 and the tyrosine kinase TrkA, but direct biochemical evidence for such an association had been lacking. In this report, we demonstrate the existence of such a gp75-TrkA complex by a copatching technique. Gp75 on the surface of intact cells is patched with an anti-gp75 antibody and fluorescent secondary antibody, the cells are then fixed to prevent further antibody-induced redistributions, and the distribution of TrkA is probed with and anti-TrkA antibody and fluorescent secondary antibody. We utilize a baculovirus-insect cell expression of wild-type and mutated NGF receptors. TrkA and gp75 copatch in both the absence and presence of NGF. The association is specific, since gp75 does not copatch with other tyrosine kinase receptors, including TrkB, platelet-derived growth factor receptor-beta, and Torso (Tor). To determine which domains of TrkA are required for copatching, we used a series of TrkA-Tor chimeric receptors and show that the extracellular domain of TrkA is sufficient for copatching with gp75. A chimeric receptor with TrkA transmembrane and intracellular domains show partial copatching with gp75. Deletion of the intracellular domain of g...Continue Reading

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Citations

Jun 26, 1999·Microscopy Research and Technique·P Casaccia-BonnefilM V Chao
Feb 17, 2001·Journal of Cellular Biochemistry·D A BulsecoA H Ross
Sep 5, 2002·Journal of Neuroscience Research·Hisanori ItoShoei Furukawa
Dec 14, 1999·Molecular Neurobiology·E J CoulsonP F Bartlett
Jul 1, 1997·Trends in Neurosciences·D E Bredesen, S Rabizadeh
Aug 10, 2002·Progress in Neurobiology·Philippe P Roux, Philip A Barker
Mar 8, 2000·Progress in Neurobiology·G L Barrett
Dec 18, 2001·Pediatric Neurology·N F Schor
Feb 16, 2000·Journal of Dermatological Science·C Pincelli, A Marconi
Apr 1, 1997·Current Opinion in Cell Biology·D R Kaplan, F D Miller
Jan 21, 2001·The Journal of Biological Chemistry·P S MischelL F Reichardt
Jun 11, 2002·The Journal of Biological Chemistry·Jérôme JullienBrian B Rudkin
Aug 25, 2001·Annual Review of Neuroscience·M V SofroniewW C Mobley
Oct 5, 2007·Pharmacology & Therapeutics·V Freund-Michel, N Frossard
Aug 21, 2012·Journal of Neuroscience Research·Hrishikesh M MehtaKenneth E Neet
Dec 17, 2005·Molecular and Cellular Neurosciences·Yangzhou DuCheryl F Dreyfus
Dec 28, 2002·Journal of Neuroscience Research·Mahesh B LachyankarAlonzo H Ross
Oct 28, 2014·Growth Factors·Maria Cristina CaroleoErika Cione
Sep 21, 2000·Molecular and Cellular Neurosciences·K AgermanP Ernfors
Sep 30, 1998·The European Journal of Neuroscience·G M RossR J Riopelle
Dec 14, 1999·The Journal of Biological Chemistry·C S HuangW C Mobley
Oct 11, 2007·Chemical Reviews·Aurore Loudet, Kevin Burgess
Oct 28, 2004·Journal of Cell Science·Matthias HartmannVolkmar Lessmann
Jul 4, 1998·Brain Research. Brain Research Reviews·M ChaoS O Yoon

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