The neutral red release assay: a review

Alternatives to Laboratory Animals : ATLA
Valérie Zuang

Abstract

The neutral red release (NRR) assay is a cytotoxicity test that can be used to measure the immediate toxic effects of test substances on the cell membrane, resulting in the leaking of intracellular contents. The assay has already been used for several years to evaluate the cytotoxicities of various kinds of products, such as cosmetics, pharmaceuticals, industrial chemicals and household products. It has undergone in-house validation by many companies, and has been found to be particularly useful for identifying substances that are potentially capable of causing adverse reactions on coming into brief contact with the eye or the skin at relatively high concentrations, such as might occur in an adventitious splash into the eye or onto the skin, followed by a quick rinse. Because of the relatively long existence of the NRR assay, its practicality and its proven usefulness for particular purposes, ECVAM decided to review the status of the method, in order to decide whether prevalidation and formal validation studies on the test might be profitable. The review of the status of the test was carried out by performing a comprehensive review of the literature, and by conducting a survey involving companies and institutes with experience ...Continue Reading

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Citations

Feb 6, 2021·In Vitro Cellular & Developmental Biology. Animal·Yulia Kaluzhny, Mitchell Klausner
Apr 9, 2021·Cutaneous and Ocular Toxicology·Amy J ClippingerAnna B Lowit
Jun 3, 2021·International Journal of Molecular Sciences·Andrés TabernillaMathieu Vinken
Oct 15, 2021·Journal of Applied Toxicology : JAT·Noémie BonneauFrançoise Brignole-Baudouin

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