The Neutralizing Antibody Response Elicited by Tembusu Virus Is Affected Dramatically by a Single Mutation in the Stem Region of the Envelope Protein

Frontiers in Microbiology
Junfeng LvDabing Zhang

Abstract

Tembusu virus (TMUV) is a mosquito-borne flavivirus that most commonly affects adult breeder and layer ducks. However, a TMUV-caused neurological disease has also been found in ducklings below 7 weeks of age, highlighting the need to develop a safe vaccine for young ducklings. In this study, a plaque-purified PS TMUV strain was attenuated by serial passage in BHK-21 cells. Using 1-day-old Pekin ducklings as a model, the virus was confirmed to be attenuated sufficiently after 180 passages, whereas the neutralizing antibody response elicited by the 180th passage virus (PS180) was substantially impaired compared with PS. The findings suggest that sufficient attenuation results in loss of immunogenicity in the development of the live-attenuated TMUV vaccine. Comparative sequence analysis revealed that PS180 acquired one mutation (V41M) in prM and four mutations (T70A, Y176H, K313R, and F408L) in the envelope (E) protein. To identify the amino acid substitution(s) associated with loss of immunogenicity of PS180, we rescued parental viruses, rPS and rPS180, and produced mutant viruses, rPS180-M41V, rPS180-A70T, rPS180-H176Y, rPS180-R313K, rPS180-L408F, and rPS180-M5, which contained residue 41V in prM, residues 70T, 176Y, 313K, and 4...Continue Reading

References

Mar 22, 2002·Vaccine·Susana VázquezMayling Alvarez
Dec 31, 2003·Advances in Virus Research·John T Roehrig
Nov 17, 2007·Cell Host & Microbe·Theodore C PiersonMichael S Diamond
May 15, 2008·Viral Immunology·John T RoehrigAlan D T Barrett
Oct 8, 2008·Immunological Reviews·Michael S DiamondDaved H Fremont
May 22, 2009·Clinical and Vaccine Immunology : CVI·Barbara W JohnsonThomas Monath
May 8, 2010·Science·Wanwisa DejnirattisaiGavin Screaton
Jun 30, 2011·PLoS Neglected Tropical Diseases·Ruklanthi de AlwisAravinda M de Silva
Oct 18, 2011·Emerging Infectious Diseases·Zhenzhen CaoDabing Zhang
Sep 25, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·F X Heinz, Karin Stiasny
Oct 2, 2012·BMC Infectious Diseases·Kaitlin Rainwater-LovettJustin Lessler
Oct 5, 2012·Biologicals : Journal of the International Association of Biological Standardization·Marisol SimõesMarcos da Silva Freire
Oct 6, 2012·The Veterinary Journal·Akihiko Maeda, Junko Maeda
Jan 17, 2013·Nature Reviews. Microbiology·Mehul S SutharMichael Gale
Nov 12, 2013·Veterinary Microbiology·X Y SunD M Hao
Oct 10, 2014·Avian Pathology : Journal of the W.V.P.A·Zalán Gábor HomonnayVilmos Palya
Jan 18, 2015·Progress in Molecular Biology and Translational Science·Theodore C Pierson, Michael S Diamond
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Jun 25, 2015·Frontiers in Microbiology·Ning LiSidang Liu
Sep 12, 2015·Science Translational Medicine·P Robert BeattyEva Harris
Oct 17, 2015·Avian Diseases·Jian LinJie Pan
Nov 20, 2015·Emerging Infectious Diseases·Aunyaratana ThontiravongAlongkorn Amonsin
Jan 23, 2016·Antiviral Research·Guntur Fibriansah, Shee-Mei Lok
Aug 17, 2016·Veterinary Microbiology·Yunjian LuYouxiang Diao
Feb 6, 2017·Research in Veterinary Science·Lijiao ZhangJingliang Su

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Datasets Mentioned

BETA
LC095865
U073166
KT438580
KP757171
JN036395
KT452794
AF091020
AF202541
KT876991
AB110494

Methods Mentioned

BETA
PCR
Transfection

Software Mentioned

GraphPad
GraphPad Prism
CLUASTALW
GeneDoc
Phyre2
PyMOL

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