The neutrophil's eye-view: inference and visualisation of the chemoattractant field driving cell chemotaxis in vivo.

PloS One
Visakan KadirkamanathanS A Renshaw

Abstract

As we begin to understand the signals that drive chemotaxis in vivo, it is becoming clear that there is a complex interplay of chemotactic factors, which changes over time as the inflammatory response evolves. New animal models such as transgenic lines of zebrafish, which are near transparent and where the neutrophils express a green fluorescent protein, have the potential to greatly increase our understanding of the chemotactic process under conditions of wounding and infection from video microscopy data. Measurement of the chemoattractants over space (and their evolution over time) is a key objective for understanding the signals driving neutrophil chemotaxis. However, it is not possible to measure and visualise the most important contributors to in vivo chemotaxis, and in fact the understanding of the main contributors at any particular time is incomplete. The key insight that we make in this investigation is that the neutrophils themselves are sensing the underlying field that is driving their action and we can use the observations of neutrophil movement to infer the hidden net chemoattractant field by use of a novel computational framework. We apply the methodology to multiple in vivo neutrophil recruitment data sets to de...Continue Reading

References

Feb 1, 1971·Journal of Theoretical Biology·E F Keller, L A Segel
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Mar 24, 2004·Current Opinion in Cell Biology·Carole A Parent
Dec 21, 2005·Journal of Leukocyte Biology·William G TharpMark C Poznansky
Feb 25, 2006·Nature Reviews. Immunology·Carl Nathan
Aug 24, 2006·Blood·Stephen A RenshawMoira K B Whyte
Sep 12, 2006·Journal of Leukocyte Biology·Jonathan R MathiasAnna Huttenlocher
Nov 13, 2007·Neural Networks : the Official Journal of the International Neural Network Society·Stephen A BillingsMichael A Balikhin
Dec 14, 2007·Experimental Lung Research·Stephen A RenshawMoira K B Whyte
Jul 16, 2008·Journal of Mathematical Biology·T Hillen, K J Painter
Apr 23, 2009·PloS One·Leonard Bosgraaf, Peter J M Van Haastert
Jul 21, 2009·Biochemical Society Transactions·Jane S Martin, Stephen A Renshaw
Jul 25, 2009·Bioinformatics·Victor OlariuVisakan Kadirkamanathan
Sep 19, 2009·Methods in Molecular Biology·Jonathan R MathiasAnna Huttenlocher
Mar 3, 2010·Annual Review of Biophysics·Kristen F SwaneyPeter N Devreotes
May 26, 2010·Nature Reviews. Immunology·Oliver Soehnlein, Lennart Lindbom
Feb 2, 2011·Nature Reviews. Microbiology·Steven L PorterJudith P Armitage
Feb 19, 2011·NeuroImage·D R FreestoneV Kadirkamanathan
May 10, 2011·PLoS Computational Biology·Alexandra Jilkine, Leah Edelstein-Keshet
Jul 13, 2011·Methods in Molecular Biology·Philip M ElksStephen A Renshaw
Oct 31, 2014·Nature Reviews. Molecular Cell Biology·Karl E Kadler

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Citations

Nov 15, 2018·PloS One·Savannah L LoganRaghuveer Parthasarathy
Mar 20, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sofia de OliveiraAngelo Calado
Jan 14, 2019·Journal of Imaging·José Alonso Solís-LemusConstantino Carlos Reyes-Aldasoro

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