The New Age of Cell-Free Biology.

Annual Review of Biomedical Engineering
Vincent Noireaux, Allen P Liu

Abstract

The cell-free molecular synthesis of biochemical systems is a rapidly growing field of research. Advances in the Human Genome Project, DNA synthesis, and other technologies have allowed the in vitro construction of biochemical systems, termed cell-free biology, to emerge as an exciting domain of bioengineering. Cell-free biology ranges from the molecular to the cell-population scales, using an ever-expanding variety of experimental platforms and toolboxes. In this review, we discuss the ongoing efforts undertaken in the three major classes of cell-free biology methodologies, namely protein-based, nucleic acids-based, and cell-free transcription-translation systems, and provide our perspectives on the current challenges as well as the major goals in each of the subfields.

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Citations

Nov 22, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Alessandro GroazAllen P Liu
Dec 15, 2020·Current Opinion in Biotechnology·Bryan R LennemanSamuel Kilcher
Nov 19, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Xiaomin QianBrigitte Städler
Aug 11, 2020·Synthetic and Systems Biotechnology·Stephanie D ColeMatthew W Lux
Mar 31, 2021·Cell·James F PelletierElizabeth A Strychalski
May 21, 2021·Journal of the American Chemical Society·Jacob A Vance, Neal K Devaraj

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