PMID: 11914125Mar 27, 2002Paper

The new anti-actin agent dihydrohalichondramide reveals fenestrae-forming centers in hepatic endothelial cells

BMC Cell Biology
Filip BraetEddie Wisse

Abstract

Liver sinusoidal endothelial cells (LSECs) react to different anti-actin agents by increasing their number of fenestrae. A new structure related to fenestrae formation could be observed when LSECs were treated with misakinolide. In this study, we investigated the effects of two new actin-binding agents on fenestrae dynamics. High-resolution microscopy, including immunocytochemistry and a combination of fluorescence- and scanning electron microscopy was applied. Halichondramide and dihydrohalichondramide disrupt microfilaments within 10 minutes and double the number of fenestrae in 30 minutes. Dihydrohalichondramide induces fenestrae-forming centers, whereas halichondramide only revealed fenestrae-forming centers without attached rows of fenestrae with increasing diameter. Correlative microscopy showed the absence of actin filaments (F-actin) in sieve plates and fenestrae-forming centers. Comparable experiments on umbilical vein endothelial cells and bone marrow sinusoidal endothelial cells revealed cell contraction without the appearance of fenestrae or fenestrae-forming centers. (I) A comparison of all anti-actin agents tested so far, revealed that the only activity that misakinolide and dihydrohalichondramide have in common i...Continue Reading

Citations

Jul 14, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tatsuya TazakiHiroaki Honda
Jul 30, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Filip BraetPeter Frederik
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Filip BraetDavid G Le Couteur
Nov 30, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·Filip Braet
Apr 13, 2006·World Journal of Gastroenterology : WJG·Masaya SaitoFilip Braet
Jan 9, 2009·American Journal of Physiology. Renal Physiology·Simon C Satchell, Filip Braet
Aug 24, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bartlomiej ZapotocznyMarek Szymonski
Dec 30, 2020·Marine Drugs·Conxita Avila, Carlos Angulo-Preckler
Jan 13, 2006·Journal of Molecular Biology·Kunio HirataHideo Kigoshi

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