The newly nonsporulated characterization of an Aspergillus fumigatus isolate from an immunocompetent patient and its clinic indication

Fungal Genetics and Biology : FG & B
Caiyun ZhangHong Sang

Abstract

Aspergillus fumigatus (A. fumigatus) commonly produces abundant and heavily melanized infectious conidia, which are the primary agents that cause invasive aspergillosis (IA) in immunocompromised patients. We isolated a white nonsporulating A. fumigatus strain (A1j) from an immunocompetent patient. It was identified by histopathological examination and morphological observation, and subsequently confirmed by DNA sequencing of internal transcribed spacer (ITS) regions and partial β-tubulin genes. Neither a long waiting time nor passage on various medium types could stimulate the formation of spores and pigment. No significant relative difference was found in sensitivity to antifungal agents or cell wall destabilizing reagents, as compared to wild-type A. fumigatus Af293. Nevertheless, A1j was hypovirulent in the immunosuppressed mice model, consistent with the good result in our patient. RNA deep-sequencing analysis (RNA-seq) revealed that hundreds of transcripts were significantly dysregulated, including those related to pigmentation and sporulation. qRT-PCR confirmed the anergic state of key regulator brlA for sporulation under the induction of conidiation conditions, but without mutation. To the best of our knowledge, this is ...Continue Reading

References

Jan 31, 1998·Molecular Microbiology·H F TsaiK J Kwon-Chung
Jun 18, 2002·Chest·Ayman O Soubani, Pranatharthi H Chandrasekar
Feb 19, 2004·Journal of Medical Microbiology·Sirida YoungchimAndrew J Hamilton
Mar 30, 2004·Antimicrobial Agents and Chemotherapy·S Arunmozhi BalajeeKieren A Marr
Aug 18, 2004·The Journal of Biological Chemistry·Isao FujiiYutaka Ebizuka
Feb 8, 2005·Journal of Clinical Microbiology·Margaret E KatzBrian F Cheetham
Sep 29, 2005·Antimicrobial Agents and Chemotherapy·Juan L Rodriguez-TudelaManuel Cuenca-Estrella
Dec 8, 2005·Journal of Clinical Microbiology·S Arunmozhi BalajeeKieren A Marr
Feb 21, 2006·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·G Chamilos, D P Kontoyiannis
Oct 13, 2006·Eukaryotic Cell·Jae-Hyung Mah, Jae-Hyuk Yu
Oct 13, 2006·Eukaryotic Cell·S Arunmozhi BalajeeKieren A Marr
May 26, 2007·QJM : Monthly Journal of the Association of Physicians·O S Zmeili, A O Soubani
Jan 24, 2008·Antimicrobial Agents and Chemotherapy·Laura Alcazar-FuoliJuan L Rodriguez-Tudela
May 21, 2008·Studies in Mycology·S A BalajeeR A Samson
Jun 17, 2008·Eukaryotic Cell·Philipp S SchmalhorstFrançoise H Routier
Nov 26, 2008·Applied and Environmental Microbiology·Jeannette Schmaler-RipckeAxel A Brakhage
Sep 16, 2009·Emerging Infectious Diseases·Donald C VinhSteven M Holland
May 8, 2010·Fungal Genetics and Biology : FG & B·Thierry FontaineJean-Paul Latgé
Nov 12, 2010·Medical Mycology·Françoise SymoensJean-Philippe Bouchara
Apr 23, 2011·BMC Microbiology·Rita SerranoRicardo Araujo
Jul 26, 2011·Current Opinion in Microbiology·Donald C Sheppard
Sep 2, 2011·European Respiratory Review : an Official Journal of the European Respiratory Society·M KoushaA O Soubani
Sep 20, 2012·Transplant Infectious Disease : an Official Journal of the Transplantation Society·A ZbindenN J Mueller
May 8, 2013·Eukaryotic Cell·Sameira S SwilaimanPaul S Dyer
Jun 14, 2013·Medical Mycology Journal·Shigeru Fujita
Aug 8, 2013·Journal of Immunotoxicology·Amanda D BuskirkDonald H Beezhold
Nov 19, 2013·Fungal Genetics and Biology : FG & B·Hechun JiangLing Lu
May 16, 2014·Journal of Clinical Microbiology·Reeti KhareNancy L Wengenack
Aug 8, 2014·Journal of Clinical Microbiology·Janyce A SuguiKyung J Kwon-Chung

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Citations

Jun 28, 2015·Fungal Genetics and Biology : FG & B·Chengshu WangKe-Qin Zhang
Jun 1, 2017·Frontiers in Microbiology·Guillaume Desoubeaux, Carolyn Cray
Apr 10, 2019·Proceedings. Biological Sciences·Jianhua ZhangAlfons J M Debets

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