PMID: 9177162Jun 10, 1997Paper

The NG domain of the prokaryotic signal recognition particle receptor, FtsY, is fully functional when fused to an unrelated integral membrane polypeptide

Proceedings of the National Academy of Sciences of the United States of America
A ZelaznyEitan Bibi

Abstract

Recent studies have revealed that Escherichia coli possesses an essential targeting system for integral membrane proteins, similar to the mammalian signal recognition particle (SRP) machinery. One essential protein in this system is FtsY, a homologue of the alpha-subunit of the mammalian SRP-receptor (SR-alpha). However, E. coli does not possess a close homologue of the integral membrane protein SR-beta, which anchors SR-alpha to the membrane. Moreover, although FtsY can be found as a peripheral membrane protein, the majority is found soluble in the cytoplasm. In this study, we obtained genetic and biochemical evidence that FtsY must be targeted to the membrane for proper function. We demonstrate that the essential membrane targeting activity of FtsY is mediated by a 198-residue-long acidic N-terminal domain. This domain can be functionally replaced by unrelated integral membrane polypeptides, thus avoiding the need for specific FtsY membrane targeting factors. Therefore, the N terminus of FtsY constitutes an independent domain, which is required only for the targeting of the C-terminal NG domain of FtsY to the membrane.

References

Aug 1, 1992·Current Opinion in Cell Biology·J Nunnari, P Walter
Apr 1, 1990·Molecular Microbiology·D R Gill, G P Salmond
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E Bibi, H R Kaback
Dec 1, 1987·Molecular & General Genetics : MGG·D R Gill, G P Salmond
Oct 25, 1985·Science·W T Wickner, H F Lodish

❮ Previous
Next ❯

Citations

Oct 11, 2007·Current Microbiology·Xue-Ling ShenYong-Quan Li
Jun 19, 2009·Current Microbiology·Hui-Jun DongYong-Quan Li
May 16, 2002·Biochimica Et Biophysica Acta·Irina V Shepotinovskaya, Douglas M Freymann
Mar 10, 2001·Trends in Microbiology·J Eichler, R Moll
Mar 17, 2001·Trends in Biochemical Sciences·E BibiA Zelazny
Mar 19, 2009·The Journal of Biological Chemistry·Naomi J MartyRobyn L Goforth
Sep 23, 2003·Acta Crystallographica. Section D, Biological Crystallography·Irina V ShepotinovskayaDouglas M Freymann
Mar 20, 2004·European Journal of Biochemistry·Tovit LichiJerry Eichler
Jun 8, 2001·Annual Review of Biochemistry·R J KeenanP Walter
Dec 15, 2007·Annual Review of Biochemistry·Arnold J M Driessen, Nico Nouwen
Oct 27, 2004·Bioscience, Biotechnology, and Biochemistry·Kunio YamaneHiroshi Kakeshita
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·A A Herskovits, E Bibi
Mar 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shu-ou Shan, Peter Walter
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Adnan HasonaL Jeannine Brady
Apr 5, 2005·Archaea : an International Microbiological Journal·Sonja M KoningArnold J M Driessen
Oct 14, 2009·Cellular and Molecular Life Sciences : CMLS·Jijun YuanRoss E Dalbey
Jul 31, 2010·Archaea : an International Microbiological Journal·Christian Zwieb, Shakhawat Bhuiyan
Nov 12, 2013·Applied and Environmental Microbiology·Annereinou R DijkstraJeroen Hugenholtz
Jun 10, 2000·European Journal of Biochemistry·J Eichler
Aug 23, 2006·The Journal of Cell Biology·Sandra AngeliniHans-Georg Koch
Nov 18, 2011·Trends in Biochemical Sciences·Eitan Bibi
Oct 14, 2008·Biochemical and Biophysical Research Communications·Shifei ChenXin Chen
Dec 14, 2005·Journal of Structural Biology·Talal GarianiA Elisabeth Sauer-Eriksson
Mar 30, 2010·Biochimica Et Biophysica Acta·Doron Calo, Jerry Eichler
Aug 5, 2010·Biochimica Et Biophysica Acta·Eitan Bibi
Feb 19, 2008·Journal of Molecular Biology·Benjamin WeicheHans-Georg Koch
Jun 30, 2016·Molecular Microbiology·Albena DraychevaWolfgang Wintermeyer
Dec 31, 1997·FEBS Letters·E de LeeuwJ Luirink
Nov 24, 1999·The Journal of Biological Chemistry·J S Millman, D W Andrews
Sep 15, 2009·Journal of Bacteriology·Jonas BürkHans-Georg Koch
Dec 21, 2000·Molecular Microbiology·A A HerskovitsE Bibi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.