The NH2-terminal domain of the human T-cell leukemia virus type 1 capsid protein is involved in particle formation

Journal of Virology
F RayneR Z Mamoun

Abstract

The human immunodeficiency virus type 1 (HIV-1) and human T-cell leukemia virus type 1 (HTLV-1) capsid proteins (CA) display similar structures formed by two independently folded N-terminal (NTD) and C-terminal (CTD) domains. To characterize the functions harbored by the HTLV-1 CA domains in particle formation, 12 sites scattered throughout the protein were mutated. The effects of the mutations on Gag membrane binding, proteolytic processing, and virus-like particle secretion were analyzed. It appears that the NTD is the major partner of indirect or direct Gag-Gag interactions. In particular, most of the NTD mutations impaired virion morphogenesis, and no mutation located in the NTD could be fully rescued by coexpression of wild-type Gag. In contrast, the CTD seems not to be involved in Gag-Gag interactions. Nevertheless, an unknown function required for particle formation is located in the CTD. Thus, despite an overall structural similarity between the HIV-1 and HTLV-1 CA proteins, their NTDs and CTDs exhibit different functions.

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Citations

Feb 2, 2016·Viruses·Jessica L MartinLouis M Mansky
Dec 10, 2013·Journal of Molecular Biology·Keir H FogartyJoachim D Mueller
Jul 11, 2014·Frontiers in Microbiology·José O MaldonadoLouis M Mansky
Dec 1, 2004·The Journal of Biological Chemistry·Fadila BouamrCarol A Carter
Aug 29, 2019·ACS Omega·Denzelle L LyngdohTimir Tripathi

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