The NH2-terminal structures of human and rat liver microsomal NADH-cytochrome b5 reductases

Journal of Biochemistry
K MurakamiT Miyata

Abstract

Detergent-solubilized NADH-cytochrome b5 reductase was purified from human liver microsomes. Both the purified enzyme and the membrane-binding domain isolated from the purified enzyme were determined to be modified at the NH2-terminal amino acid, glycine, by myristic acid in an amide form. Myristic acid was identified as a methyl ester by gas chromatography after the acid methanolysis of the purified enzyme and the NH2-terminal peptide. The NH2-terminal structure of the membrane-binding domain was determined to be CH3(CH2)12-CO-Gly-Ala-Gln-Leu-Ser-Thr-Leu-Gly-His-Met-Val-Leu-Phe-Pro-Va l- Trp-Phe-Leu-Tyr-Ser-Leu-Leu-Met-Lys. The sequence from Leu-7 to Lys-24 completely coincided with that deduced from the base sequence of complementary DNA (cDNA) from human placenta (Yubisui, T. et al. (1987) Proc. Natl. Acad. Sci. U.S. 84, 3609-3613). The NH2-terminal structure of the detergent-solubilized enzyme from rat liver microsomes was also analyzed for comparison with that of human liver microsomal enzyme. The NH2-terminal myristic acid and the first 7 amino acids of the membrane-binding domains of human, rat, and steer liver microsomal enzymes are completely conserved, and more than 70% homology was observed over the whole membrane-bi...Continue Reading

Citations

Oct 26, 2001·Protein Expression and Purification·M J Barber, G B Quinn
May 8, 2001·Archives of Biochemistry and Biophysics·C C Marohnic, M J Barber
Jun 27, 1997·Biochemical and Biophysical Research Communications·M DuM Takeshita

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