The Ni(II)-binding properties of the metallochaperone SlyD

Journal of the American Chemical Society
Harini KaluarachchiDeborah B Zamble

Abstract

Metallochaperones are essential for the safe and targeted delivery of necessary yet toxic metal cofactors to their respective protein partners. In this study we examine the nickel-binding properties of the Escherichia coli protein SlyD, a factor that contributes to optimal nickel accumulation in this organism. This protein is also required for E. coli energy metabolism because it participates in the nickel insertion step during [Ni-Fe]-hydrogenase metallocenter assembly. Our study demonstrates that SlyD is a multiple nickel ion binding protein. The analysis of noncovalent metal-protein complexes via electrospray ionization mass spectrometry revealed that SlyD binds up to seven nickel ions in a noncooperative manner with submicromolar affinity (<2 microM, upper limit) and that the protein exists in a dynamic mixture of metalloforms that is dependent on the availability of nickel ions in solution. Structural analysis indicates that this metallochaperone undergoes small but distinct changes in the structure upon metal binding and that the nickel-binding sites are assembled through beta-turn formation. Although the C-terminal metal-binding domain is primarily responsible for metal chelation, we find that metal binding also perturbs...Continue Reading

References

Mar 1, 1992·Protein Science : a Publication of the Protein Society·A Perczel, G D Fasman
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G O TanK O Hodgson
Nov 10, 1981·Biochemistry·M VasákB L Vallee
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·C FuR J Maier
Jun 1, 1993·Journal of Bacteriology·W D Roof, R Young
Jun 20, 1997·The Journal of Biological Chemistry·S HottenrottJ U Rahfeld
Feb 20, 1999·Cell·U JakobJ C Bardwell
May 6, 1999·Cellular and Molecular Life Sciences : CMLS·S F Göthel, M A Marahiel
Jun 26, 1999·Journal of Toxicology. Clinical Toxicology·D G Barceloux
May 19, 2000·The Journal of Biological Chemistry·T V O'Halloran, V C Culotta
May 23, 2002·Biochemistry·Faizah Al-MjeniMichael J Maroney
Jun 25, 2002·Chemistry & Biology·Amy C Rosenzweig
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger
Dec 19, 2003·Chemical Communications : Chem Comm·Jason A Halfen, Victor G Young
Feb 12, 2004·Chemical Reviews·Jason Kuchar, Robert P Hausinger
Aug 4, 2004·Biochemistry·Sheila C WangDeborah B Zamble
Aug 4, 2004·Biochemistry·Stephanie L Bloom, Deborah B Zamble
Aug 27, 2004·Journal of Molecular Biology·Hyung-Sik WonH Jane Dyson
Nov 3, 2004·Analytical Biochemistry·Anelia AtanassovaDeborah B Zamble
Dec 1, 2004·The Journal of Biological Chemistry·Jie Wei ZhangDeborah B Zamble
Feb 9, 2005·Biochimica Et Biophysica Acta·Peep PalumaaRannar Sillard
Sep 7, 2005·Biochemistry·Michael R LeachDeborah B Zamble
Nov 18, 2005·The Journal of Biological Chemistry·Virginie MartinMarek Michalak
Jan 4, 2006·Biochemistry·Christian ScholzFranz Xaver Schmid
Oct 24, 2006·Journal of Inorganic Biochemistry·Kelly E Rigby Duncan, Martin J Stillman
Nov 7, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Maria T SalgadoMartin J Stillman
Nov 10, 2006·Advances in Microbial Physiology·August BöckMatthew C Posewitz
Jan 12, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Lucia Forzi, R Gary Sawers
Feb 6, 2007·Current Opinion in Chemical Biology·Michael R Leach, Deborah B Zamble
Apr 3, 2007·Journal of Molecular Biology·Thomas A KnappeFranz X Schmid
Apr 12, 2007·The Journal of Biological Chemistry·Michael R LeachDeborah B Zamble

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Citations

Nov 4, 2011·Biochemistry·Harini KaluarachchiDeborah B Zamble
Nov 17, 2011·Biochemistry·Harini KaluarachchiDeborah B Zamble
Sep 14, 2012·Biochemistry·Khadine A HigginsMichael J Maroney
Mar 25, 2014·Chemical Reviews·Wolfgang LubitzEdward Reijerse
Dec 27, 2012·Biophysical Chemistry·Michael Kovermann, Jochen Balbach
Feb 27, 2016·Biochemistry·Michael J Lacasse, Deborah B Zamble
Dec 1, 2011·Biomolecular Concepts·Bo Hou, Thomas Brüser
Feb 9, 2017·Scientific Reports·Amit Kumar, Jochen Balbach
Mar 30, 2017·Metallomics : Integrated Biometal Science·Mozhgan Khorasani-MotlaghDeborah B Zamble
May 6, 2010·Natural Product Reports·Harini KaluarachchiDeborah B Zamble
Feb 14, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mozhgan Khorasani-MotlaghDeborah B Zamble
Feb 6, 2020·Protein Science : a Publication of the Protein Society·Marila Alfano, Christine Cavazza
Apr 16, 2013·Biological Chemistry·Michael KovermannJochen Balbach
Mar 7, 2020·Inorganics·Khadine Higgins
Jan 15, 2021·PLoS Pathogens·Milica DenicHilde de Reuse
Nov 23, 2019·Protein Expression and Purification·Navjit K PaulDeborah B Zamble
Jul 20, 2021·The Journal of Physical Chemistry. B·Abhigyan SenguptaMatthias Rief
Dec 20, 2011·Journal of Inorganic Biochemistry·Danuta WitkowskaHenryk Kozlowski
Dec 29, 2021·Metallomics : Integrated Biometal Science·Stéphane L Benoit, Robert J Maier

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