The nitrative and oxidative stress in blood platelets isolated from breast cancer patients: the protectory action of aronia melanocarpa extract

Platelets
Magdalena KedzierskaJanusz Piekarski

Abstract

Since mechanisms involved in the relationship between oxidative stress and breast cancer are still unclear, the aim of our present study was to evaluate oxidative/nitrative modifications of blood platelet proteins by measuring the level of biomarkers of oxidative/nitrative stress such as carbonyl groups, thiol groups and 3-nitrotyrosine in proteins in patients with benign breast diseases and in patients with invasive breast cancer, and compare with the control group. Levels of carbonyl groups and 3-nitrotyrosine residues in platelet proteins were measured by ELISA and a competition ELISA, respectively. The method with 5,5′-dithio-bis(2-nitro-benzoic acid) has been used to analyse free thiol groups in platelet proteins. Patients were hospitalized in the Department of Oncological Surgery, Medical University of Lodz, Poland. Exogenous antioxidants reduce oxidative stress, therefore we also investigated in a model system in vitro the effects of a polyphenol rich extract of Aronia melanocarpa (Rosaceae, final concentration of 50 µg/ml, 5 min, 37°C) on modified blood platelet proteins as well from patients with breast cancer and from the healthy group. We demonstrated in platelet proteins from patients with invasive breast cancer a h...Continue Reading

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Citations

Jan 30, 2013·International Journal of Biological Macromolecules·Michał BijakPaweł Nowak
Oct 1, 2010·Trends in Cardiovascular Medicine·Nicoleta AlexandruAdriana Georgescu
Apr 12, 2016·Phytotherapy Research : PTR·Mohammed El Haouari, Juan A Rosado
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May 3, 2011·Molecular and Cellular Biochemistry·Magdalena KedzierskaJanusz Piekarski
Apr 18, 2018·Frontiers in Pharmacology·Beata Olas
Jul 15, 2020·International Journal of Molecular Sciences·Elena MasselliGiuliana Gobbi

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