The nitric oxide donor sodium nitroprusside inhibits mineralization in ATDC5 cells

Calcified Tissue International
L F A HuitemaC H A van de Lest

Abstract

The aim of this study was to test whether the nitric oxide (NO) donor sodium nitroprusside (SNP) has an effect on mineralization in ATDC5 cells. Mineralization in ATDC5 cell culture was induced by addition of beta-glycerophosphate or inorganic phosphate, visualized by staining precipitated calcium with an alizarin red stain, and quantified using atomic absorption spectrometry. SNP was shown to inhibit the mineralization of ADTC5 cells. This inhibition was not affected by inhibitors of guanylyl cyclase nor mimicked by a cyclic guanosine monophosphate (cGMP) analog. Furthermore, SNP did not inhibit phosphate uptake or inhibit apoptosis in ATDC5 cells. These findings indicate that SNP can specifically inhibit matrix mineralization via a cGMP-independent pathway and that the effect is not mediated by inhibition of phosphate transport or apoptosis. These results suggest a preventive role of NO in premature or pathological mineralization.

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Citations

Oct 10, 2009·Human Reproduction·S C MizrakA M M van Pelt
Mar 12, 2008·Calcified Tissue International·Rubens Ferreira de AlbuquerqueJoão Paulo Mardegan Issa
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Robert S JansenKoen van de Wetering
Jul 11, 2006·European Journal of Pharmacology·Leonie F A HuitemaArie B Vaandrager
Aug 19, 2007·The Journal of Biological Chemistry·Dongwei FanYongfeng Shang

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