The nitric oxide precursor L-arginine reduces expression of hyaluronan synthase in experimental vein bypass grafts

The Journal of Surgical Research
J B DattiloR G Makhoul

Abstract

The success of vascular bypass procedures is limited by the development of intimal hyperplasia (IH). The nitric oxide (NO) precursor, L-arginine (L-ARG) significantly reduces IH in both arteries and experimental vein grafts; however, the precise mechanism has yet to be elucidated. Hyaluronan synthase-1 (HAS-1) is one of the two enzymes believed to be responsible for making hyaluronan, a key component extracellular matrix composition. To determine how L-ARG supplementation affects the gene expression of HAS-1 in experimental vein grafts. Thirty-four male New Zealand white rabbits were divided into three groups: control (no operation, regular chow and water, n = 4); L-ARG supplemented (n = 15); and no L-ARG (n = 15). The latter two groups underwent a right interposition carotid bypass using jugular vein. Vein grafts were harvested at 7, 14, and 21 days after surgery. Ribonuclease protection assays were performed using 32P-labeled riboprobes for HAS-1 and 18S rRNA as an internal control and expressed as a ratio (HAS-1/rRNA). There was a significant rise in HAS-1 expression in the vein grafts 7 (1.57 +/- 0.5), 14 (0.7 +/- 0.2), and 21 days (2.82 +/- 0.7) after grafting compared to control (0.14 +/- 0.08) (P < 0.05). L-ARG-supplemen...Continue Reading

Citations

Mar 12, 2015·The Journal of Surgical Research·Enrico A DuruMark G Davies
Aug 8, 2007·Journal of Vascular Surgery·Sadaf S AhanchiMelina R Kibbe
Aug 20, 2005·Journal of Cellular Physiology·Thomas S WilkinsonThomas N Wight

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