The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway

Biological Research
Xiao-Ling NiuJing-Xiang Li

Abstract

Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorectal cancer and explored the underlying mechanism. The cell proliferation of colorectal cancer cells was detected by cell counting kit-8 (CCK8) assay, cell migration and invasion were assessed by transwell assay, the apoptotic ratio of cells was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide stained and flow cytometry. The involvement of molecular mechanisms was examined by western blot. In this study, we found that NKP608 inhibited the proliferation, migration/invasion of HCT116 cells. In addition, NKP608 reduced expressions of Wnt-3a, β-catenin, Cyclin D1, and (vascular endothelial growth factor) VEGF while induced expression of E-Cadherin. Furthermore, flow cytometry analyzed that NKP608 induced apoptosis of HCT116 cells, consistently, western blotting detecting of apoptosis-related proteins revealed that NKP608 downregulated Bcl-2 while upregulated Bax and Active-Caspase-3. Taken together, our results demonstrated that NKP608 inhib...Continue Reading

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Citations

Jun 6, 2020·Reproduction in Domestic Animals = Zuchthygiene·Lina HeZhongquan Zhao
Aug 3, 2019·Journal of Physiology and Biochemistry·Hossein JavidSeyed Isaac Hashemy
Jan 21, 2021·Cancers·Simone L SchonkerenVeerle Melotte
Jul 9, 2021·Journal of Medicinal Chemistry·Rocío RecioInmaculada Fernández

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

SPSS
QUANTITY ONE

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