The NlpD lipoprotein is a novel Yersinia pestis virulence factor essential for the development of plague.

PloS One
Avital TidharEmanuelle Mamroud

Abstract

Yersinia pestis is the causative agent of plague. Previously we have isolated an attenuated Y. pestis transposon insertion mutant in which the pcm gene was disrupted. In the present study, we investigated the expression and the role of pcm locus genes in Y. pestis pathogenesis using a set of isogenic surE, pcm, nlpD and rpoS mutants of the fully virulent Kimberley53 strain. We show that in Y. pestis, nlpD expression is controlled from elements residing within the upstream genes surE and pcm. The NlpD lipoprotein is the only factor encoded from the pcm locus that is essential for Y. pestis virulence. A chromosomal deletion of the nlpD gene sequence resulted in a drastic reduction in virulence to an LD(50) of at least 10(7) cfu for subcutaneous and airway routes of infection. The mutant was unable to colonize mouse organs following infection. The filamented morphology of the nlpD mutant indicates that NlpD is involved in cell separation; however, deletion of nlpD did not affect in vitro growth rate. Trans-complementation experiments with the Y. pestis nlpD gene restored virulence and all other phenotypic defects. Finally, we demonstrated that subcutaneous administration of the nlpD mutant could protect animals against bubonic and...Continue Reading

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Citations

Jun 15, 2011·Applied Microbiology and Biotechnology·Jason A RosenzweigAshok K Chopra
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Methods Mentioned

BETA
electrophoresis
light microscopy
ELISA
environmental stress
PCR
Protein Assay
Gel Filtration

Software Mentioned

Mauve
GeneScanR Analysis

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