The Nodulin 26-like intrinsic membrane protein OsNIP3;2 is involved in arsenite uptake by lateral roots in rice.

Journal of Experimental Botany
Yi ChenFang-Jie Zhao

Abstract

Previous studies have shown that the Nodulin 26-like intrinsic membrane protein (NIP) Lsi1 (OsNIP2;1) is involved in arsenite [As(III)] uptake in rice (Oryza sativa). However, the role of other rice NIPs in As(III) accumulation in planta remains unknown. In the present study, we investigated the role OsNIP3;2 in As(III) uptake in rice. When expressed in Xenopus laevis oocytes, OsNIP3;2 showed a high transport activity for As(III). Quantitative real-time RT-PCR showed that the expression of OsNIP3;2 was suppressed by 5 µM As(III), but enhanced by 20 and 100 µM As(III). Transgenic rice plants expressing OsNIP3;2pro-GUS showed that the gene was predominantly expressed in the lateral roots and the stele region of the primary roots. Transient expression of OsNIP3;2:GFP fusion protein in rice protoplasts showed that the protein was localized in the plasma membrane. Knockout of OsNIP3;2 significantly decreased As concentration in the roots, but had little effect on shoot As concentration. Synchrotron microfocus X-ray fluorescence showed decreased As accumulation in the stele of the lateral roots in the mutants compared with wild-type. Our results indicate that OsNIP3;2 is involved in As(III) uptake by lateral roots, but its contributi...Continue Reading

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Citations

Aug 14, 2019·Journal of Experimental Botany·Stephan Clemens
Mar 5, 2020·Journal of Experimental Botany·Chengcheng WangFang-Jie Zhao
Jul 6, 2017·Frontiers in Plant Science·Surabhi AwasthiRudra D Tripathi
Apr 5, 2020·Science China. Life Sciences·Zhizhong GongJian-Kang Zhu
Jan 27, 2020·Ecotoxicology·Daniel Gomes CoelhoJuraci Alves Oliveira
Jan 25, 2020·Plants·Tayebeh Abedi, Amin Mojiri
Jan 9, 2021·Journal of Hazardous Materials·Amos Musyoki MawiaPeisong Hu
Dec 20, 2020·Journal of Hazardous Materials·Yadira Peña-GarciaUmesh K Reddy
Oct 27, 2020·Plant Direct·Lauren WhittIvan Baxter
Nov 15, 2020·Journal of Hazardous Materials·Fenglin DengZhong-Hua Chen
Jan 21, 2021·Journal of Experimental Botany·Poonam PanchalJitender Giri
Feb 23, 2021·Frontiers in Plant Science·Sarah Tepler DrobnitchSean M Gleason
Jun 21, 2021·Chemosphere·Aditi Shreeya Bali, Gagan Preet Singh Sidhu
Jul 21, 2021·International Journal of Molecular Sciences·Sajad AliHanhong Bae
Aug 25, 2021·The Science of the Total Environment·Debojyoti MoulickSubhas Chandra Santra
Sep 16, 2021·The EMBO Journal·Qiying XiaoJeremy D Murray
Nov 14, 2021·Biological Trace Element Research·Vívian Ebeling VianaCamila Pegoraro

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