The non-conserved region of cucumopine-type Agrobacterium rhizogenes T-DNA is responsible for hairy root induction

Plant Molecular Biology
M C FaillaM Cardarelli

Abstract

The T-DNA regions of three strains of Ri plasmids 1855, 8196, 2659 (agropine, mannopine and cucumopine type respectively) share two highly conserved regions flanking a non-homologous central part. We have cloned segments of the cucumopine Ri plasmid 2659 T-DNA in the binary vector system Bin 19 and infected carrot discs with recombinant Agrobacterium strains. We show here that the central non-conserved region is crucial in hairy root induction as it is sufficient to induce rooting on the apical (auxin-rich) surface of carrot discs; in order to observe rooting on the basal (auxin-depleted) side of the discs, a longer T-DNA fragment, also encompassing part of the right conserved region, had to be utilized in conjunction with a Agrobacterium strain carrying aux genes. Differences of growth properties in culture are exhibited by roots transformed with different fragments of pRi 2659 T-DNA, although all transformed roots show the plagiotropic behaviour typical of hairy roots.

References

Nov 26, 1984·Nucleic Acids Research·M Bevan
Jan 1, 1984·Journal of Bacteriology·G A HuffmanE W Nester
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F F WhiteE W Nester
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I A OffringaP J Hooykaas
Oct 1, 1987·Molecular & General Genetics : MGG·M CardarelliP Costantino

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Citations

Jan 1, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rosa PadillaIsabelle Kerzaon
Jan 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Savio D RodriguesLaurens Pauwels

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