The non-covalent decoration of self-assembling protein fibers

Biomaterials
Zahra N MahmoudDerek N Woolfson

Abstract

The design of self-assembling fibers presents challenges in basic science, and has potential for developing materials for applications in areas such as tissue engineering. A contemporary issue in the field is the construction of multi-component, functionalized systems. Previously, we have developed peptide-based fibers, the SAF system, that comprises two complementary peptides, which affords considerable control over assembly and morphology. Here we present a straightforward route to functionalizing the SAFs with small molecules and, subsequently, other moieties. This is achieved via non-covalent recruitment of charged peptide tags, which offers advantages such as further control, reversibility, and future prospects for developing recombinant tags. We demonstrate the concept by appending fluorescent labels and biotin (and thence gold nanoparticles) to the peptides, and visualising the resulting decorated SAFs by light and electron microscopy. The peptide tags bind in the nm-mum range, and show specificity compared with control peptides, and for the SAFs over similar alpha-helix-based peptide fibers.

References

Apr 22, 2003·Nature Materials·Maxim G Ryadnov, Derek N Woolfson
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Citations

Aug 26, 2011·Biomacromolecules·Joshua Z Gasiorowski, Joel H Collier
Aug 3, 2010·Chemical Society Reviews·Derek N Woolfson, Zahra N Mahmoud
Aug 3, 2013·Physical Chemistry Chemical Physics : PCCP·Gunjan Verma, P A Hassan
Feb 27, 2013·Molecular BioSystems·Hiroshi Tsutsumi, Hisakazu Mihara
Jan 31, 2003·Journal of Biomaterials Science. Polymer Edition·Kelly R StevensWeiyuan John Kao
May 17, 2012·Biointerphases·David R Nisbet, Richard J Williams
May 10, 2013·Nanomedicine·Steven MaudeAmalia Aggeli
May 2, 2014·Chemical Society Reviews·Charlotte A E HauserIvo C Martins
Dec 18, 2015·Biomacromolecules·Jeannette E MarineJonathan Gallagher Rudick
Oct 16, 2015·Advanced Healthcare Materials·Yihua LooAnna Mitraki
Nov 2, 2011·Biotechnology Advances·Yihua LooCharlotte A E Hauser
Mar 1, 2011·Biomaterials·Zahra N MahmoudDerek N Woolfson
Feb 20, 2015·Protein Science : a Publication of the Protein Society·Toshiaki TakeiKin-ichiro Miura
Jul 19, 2012·Angewandte Chemie·Jindřich Kopeček, Jiyuan Yang
Nov 21, 2015·Chemistry & Biology·Romila D GopalanMarie-Isabel Aguilar
Apr 4, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wen LiuWilson S Meng
May 3, 2011·Biomaterials·Richard J WilliamsPatrick G Hartley
Sep 7, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Yaoying Wu, Joel H Collier
Jan 11, 2019·Chemical Communications : Chem Comm·Rie WakabayashiNoriho Kamiya
Jul 9, 2018·ACS Biomaterials Science & Engineering·Rui ZhangBret D Ulery
Jan 6, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Keisuke IkedaMinoru Nakano

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