PMID: 11900278Mar 20, 2002Paper

The non-immune RIP-Kb mouse is a useful host for islet transplantation, as the diabetes is spontaneous, mild and predictable

International Journal of Experimental Diabetes Research
Robyn M SutherlandAndrew M Lew

Abstract

Chemically-induced diabetic mice and spontaneously diabetic NOD mice have been valuable as recipients for experimental islet transplantation. However, their maintenance often requires parenteral insulin. Diabetogenic chemicals can be cytotoxic to the host's immune system and to other organs some of which are often used as the transplant site. Procurement of diabetic cohorts in the NOD mouse is problematic due to variability in the age of disease onset. We show that RIP-Kb mice, which spontaneously develop non-immune diabetes due to over-expression of the H-2Kb heavy chain in beta cells, offer many advantages as islet transplant recipients. Diabetes is predictable with a relatively narrow range of onset (4 wk) and blood glucose levels (23.0 +/- 4.0 mmol/l for 39 males at 6 weeks of age). The diabetes is mild enough so that most diabetic mice can be maintained to 40 weeks of age without parenteral insulin. This consistency of diabetes avails that outcomes of intervention can be interpreted with confidence.

Citations

May 4, 2011·Cell Transplantation·Peter D CampbellHelen E Thomas
Oct 5, 2010·Cell Transplantation·Robyn M SutherlandAndrew M Lew
Jul 9, 2004·Transplantation·Robyn M SutherlandAndrew M Lew
Jun 30, 2009·Tissue Engineering. Part a·Alan J HusseySandra J Feeney
Sep 3, 2010·Transplantation·Sarah L LondriganAndrew M Lew

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