PMID: 6722891Apr 1, 1984Paper

The nonhistone proteins of developing mammalian erythroid cells

Cell Differentiation
G J Threadgill, H R Arnstein

Abstract

Anaemic rabbit bone marrow cells, labelled with [35S]methionine, were separated at unit gravity. Chromatin was isolated from these cells and proteins were separated from DNA, using urea/salt extraction. Two-dimensional gel electrophoresis of the nonhistone proteins showed that these proteins appeared to change quantitatively but not qualitatively, with one important exception, as cell development proceeded. The one protein that did change had a molecular weight of approximately 20,000 and was very basic. This protein was synthesised at low levels in the early cells, but its synthesis was seen to increase at the polychromatic stage of cell development, just prior to nuclear condensation. Treatment of bone marrow cells with sodium butyrate was shown to increase the synthesis of this protein in the early cells.

References

Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Jan 1, 1975·Annual Review of Biochemistry·S C Elgin, H Weintraub
May 1, 1975·FEBS Letters·M N MedvedevaZ A Medvedev
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Jan 1, 1973·British Journal of Haematology·M J Denton, H R Arnstein
Apr 25, 1973·Journal of Molecular Biology·R L Seale, A I Aronson
Apr 1, 1974·Developmental Biology·F ChytilT C Splesberg
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Dec 4, 1969·Biochemical and Biophysical Research Communications·S Panyim, R Chalkley

Citations

Nov 20, 1985·Biochimica Et Biophysica Acta·G J Threadgill, H R Arnstein

Related Concepts

Metazoa
Marrow
Butyric Acids
Chromatin
Chromosomal Proteins, Non-Histone
SDS-PAGE
Colony-Forming Units, Hematopoietic
Chinchilla Rabbits
Magnesium Butyrate

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