The nonsense-mediated decay RNA surveillance pathway

Annual Review of Biochemistry
Yao-Fu ChangMiles F Wilkinson

Abstract

Nonsense-mediated mRNA decay (NMD) is a quality-control mechanism that selectively degrades mRNAs harboring premature termination (nonsense) codons. If translated, these mRNAs can produce truncated proteins with dominant-negative or deleterious gain-of-function activities. In this review, we describe the molecular mechanism of NMD. We first cover conserved factors known to be involved in NMD in all eukaryotes. We then describe a unique protein complex that is deposited on mammalian mRNAs during splicing, which defines a stop codon as premature. Interaction between this exon-junction complex (EJC) and NMD factors assembled at the upstream stop codon triggers a series of steps that ultimately lead to mRNA decay. We discuss whether these proofreading events preferentially occur during a "pioneer" round of translation in higher and lower eukaryotes, their cellular location, and whether they can use alternative EJC factors or act independent of the EJC.

References

May 1, 1992·Molecular and Cellular Biology·P LeedsM R Culbertson
Jul 1, 1989·Molecular and Cellular Biology·G UrlaubL A Chasin
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·B S Lee, M R Culbertson
Aug 24, 1995·Nature·E IzaurraldeI W Mattaj
Aug 18, 1994·Nature·D Muhlrad, R Parker
Sep 1, 1994·Molecular and Cellular Biology·J ChengL E Maquat
Oct 1, 1993·Genes & Development·R Pulak, P Anderson
Jul 15, 1997·European Journal of Biochemistry·J D Lewis, E Izaurralde
Mar 10, 1998·Immunity·S Li, M F Wilkinson
Jun 17, 1998·The EMBO Journal·R ThermannA E Kulozik
Sep 2, 1999·Human Molecular Genetics·P A Frischmeyer, H C Dietz
Dec 3, 1999·Science·M Ibba, D Söll
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·M J Luo, R Reed
Feb 26, 2000·Annual Review of Genetics·P Hilleren, R Parker
Jun 10, 2000·Molecular and Cellular Biology·A B MaderazoA Jacobson
Nov 1, 2000·Cell·T Schmelzle, M N Hall
Feb 13, 2001·Current Opinion in Genetics & Development·Y Shiloh
Feb 24, 2001·Cell·L E Maquat, G G Carmichael

❮ Previous
Next ❯

Citations

Apr 28, 2012·Cellular and Molecular Life Sciences : CMLS·Lucy W BarrettSteve D Wilton
Nov 8, 2012·Journal of Molecular Evolution·Kenneth B HoehnMohamed A F Noor
Oct 17, 2012·Plant Cell Reports·Deng PanHongbo Gao
Oct 7, 2008·Journal of Inherited Metabolic Disease·Niels GregersenPeter Bross
Jul 3, 2008·In Vitro Cellular & Developmental Biology. Animal·Joshua D O'DonnellPatricia A Kruk
Apr 3, 2009·The Journal of Physiological Sciences : JPS·Hirotaka IshiiYasuo Sakuma
Jul 23, 2013·Methods : a Companion to Methods in Enzymology·Naoto ImamachiNobuyoshi Akimitsu
Jun 20, 2012·Biochemistry·Kathleen F MittendorfCharles R Sanders
Feb 12, 2010·Nature·Stuart B SmithMichael S German
Sep 4, 2013·Nature Communications·Kei EndoHirohide Saito
Mar 23, 2011·Nature Immunology·Owen M SiggsBruce Beutler
Aug 6, 2008·Nature Reviews. Genetics·Olaf Isken, Lynne E Maquat
Jul 3, 2008·Nature Reviews. Molecular Cell Biology·Karlene A Cimprich, David Cortez
Jun 9, 2009·Nature Structural & Molecular Biology·Wai-Kin ChanMiles F Wilkinson
Feb 7, 2012·Nature Structural & Molecular Biology·Mo ChenJames L Manley
Mar 6, 2012·Nature Structural & Molecular Biology·Andrea Giovanni MurachelliElena Conti
Apr 24, 2012·Nature Structural & Molecular Biology·Roberto MeleroOscar Llorca
Jun 6, 2012·Nature Structural & Molecular Biology·Christopher J Shoemaker, Rachel Green
May 15, 2013·Nature Structural & Molecular Biology·Sébastien Durand, Jens Lykke-Andersen
May 15, 2013·Nature Structural & Molecular Biology·Simone C Rufener, Oliver Mühlemann
Oct 3, 2009·Journal of Speech, Language, and Hearing Research : JSLHR·J Bruce TomblinKirrie Ballard
Sep 25, 2009·The New England Journal of Medicine·Qian ZhangHelen C Su
Oct 15, 2010·The New England Journal of Medicine·Kimberly C WiegandDavid G Huntsman
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sascha LaubingerDetlef Weigel
May 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hao ChenJohn J Marchalonis
May 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Garabet YeretssianMaya Saleh
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eyal KamhiJoseph Sperling
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mor MeyerovichSigal Ben-Yehuda
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Aaron M Kershner, Judith Kimble
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gretel BuchwaldElena Conti
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tim FergestadBarry Ganetzky
Jan 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Noémie HaudAdam F L Hurlstone
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sebastien ApcherRobin Fåhraeus
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Whitney E PurthaDeepta Bhattacharya
Aug 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kelsey D GalimbaVerónica S Di Stilio
Aug 29, 2013·Proceedings of the National Academy of Sciences of the United States of America·Fusako UsukiShigeo Ohno
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sébastien ApcherRobin Fåhraeus
Sep 24, 2009·The Journal of Biological Chemistry·Yubin ZhangThomas W Gettys

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
footprinting
pull-down

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis