The nonsteroidal anti-inflammatory drug indomethacin induces heterogeneity in lipid membranes: potential implication for its diverse biological action.

PloS One
Yong ZhouLenard M Lichtenberger

Abstract

The nonsteroidal anti-inflammatory drug (NSAID), indomethacin (Indo), has a large number of divergent biological effects, the molecular mechanism(s) for which have yet to be fully elucidated. Interestingly, Indo is highly amphiphilic and associates strongly with lipid membranes, which influence localization, structure and function of membrane-associating proteins and actively regulate cell signaling events. Thus, it is possible that Indo regulates diverse cell functions by altering micro-environments within the membrane. Here we explored the effect of Indo on the nature of the segregated domains in a mixed model membrane composed of dipalmitoyl phosphatidyl-choline (di16:0 PC, or DPPC) and dioleoyl phosphatidyl-choline (di18:1 PC or DOPC) and cholesterol that mimics biomembranes. Using a series of fluorescent probes in a fluorescence resonance energy transfer (FRET) study, we found that Indo induced separation between gel domains and fluid domains in the mixed model membrane, possibly by enhancing the formation of gel-phase domains. This effect originated from the ability of Indo to specifically target the ordered domains in the mixed membrane. These findings were further confirmed by measuring the ability of Indo to affect the...Continue Reading

References

Oct 1, 1981·The Tohoku Journal of Experimental Medicine·M Abe, A Sevanian
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·T A ChanK W Kinzler
May 3, 2003·Nature Reviews. Molecular Cell Biology·John F Hancock
Jun 14, 2005·Biophysical Journal·Yong Zhou, Robert M Raphael
Jun 16, 2005·The Biochemical Journal·John F Hancock, Robert G Parton
Jul 6, 2005·Trends in Biochemical Sciences·Dov LichtenbergHeiko Heerklotz
Aug 11, 2005·Journal of the American Chemical Society·Sandro KellerHeiko Heerklotz
Oct 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sarah J PlowmanJohn F Hancock
Apr 21, 2006·Nature Reviews. Molecular Cell Biology·John F Hancock
Oct 13, 2006·The Journal of Pharmacy and Pharmacology·Majella E Lane, Mi-Jeong Kim
Nov 15, 2006·Annual Review of Medicine·Yong I Cha, Raymond N DuBois
Mar 14, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Marlene LúcioGerald Brezesinski
Jun 1, 2010·The Journal of Pharmacology and Experimental Therapeutics·Koji AndoNorimichi Nakahata

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Citations

Dec 19, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sreeja ChakrabortyMunna Sarkar
Mar 6, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yong ZhouLenard M Lichtenberger
Jan 25, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sreeja ChakrabortyMunna Sarkar
Jun 12, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·N NaddaS N Sanyal
Oct 13, 2015·Biochimica Et Biophysica Acta·Ana Rute NevesSalette Reis
Oct 17, 2014·Medicinal Research Reviews·Sharad Kumar Suthar, Manu Sharma
Sep 20, 2011·Seminars in Cell & Developmental Biology·Ian A Prior, John F Hancock
Oct 19, 2012·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Itsuro KazamaSatoshi Nakamichi
Oct 21, 2015·PloS One·Krishnan RaghunathanSarah L Veatch
Oct 30, 2013·The Journal of Biological Chemistry·Yong ZhouIlya Levental
Nov 2, 2019·Biochimica Et Biophysica Acta. Biomembranes·V K SharmaM Tyagi
Nov 9, 2012·Journal of the American Chemical Society·Mohan Babu BoggaraRamanan Krishnamoorti
Oct 24, 2018·The Journal of Physical Chemistry. B·P S DubeyR Mukhopadhyay
Oct 25, 2017·The Journal of Physical Chemistry. B·Amanda D Fearon, Grace Y Stokes

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Methods Mentioned

BETA
electron microscopy
fluorescence resonance
FRET
dynamic light scattering

Software Mentioned

Indo

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