PMID: 2509485Nov 1, 1989Paper

The notch gene product is a glycoprotein expressed on the cell surface of both epidermal and neuronal precursor cells during Drosophila development

The Journal of Cell Biology
K M JohansenS Artavanis-Tsakonas

Abstract

The Notch locus of Drosophila melanogaster is one of a small number of zygotically acting "neurogenic" genes involved in the correct segregation of neural from epidermal lineages during embryogenesis as well as in other postembryonic developmental events. We have generated antibody probes against three regions of the Notch protein to study the expression of Notch and begin a biochemical characterization of the protein. Consistent with predictions based on DNA sequence data, here we gather evidence showing that Notch encodes a large, glycosylated surface protein with an apparent molecular mass of 300 kD: (a) all three antibodies detect Notch on Western blots as a high molecular mass, primarily full-length product; (b) immunoelectron microscopy localizes the Notch protein to the cell membrane; and (c) lentil lectin column binding demonstrates that the protein is glycosylated, indicative of its surface protein nature. In general, the distribution of the Notch protein coincides with that of the Notch transcript determined previously by in situ hybridizations. Notch is expressed in a much wider range of tissue types than those disrupted in the neurogenic mutant, as determined by antibody localization. Early labeling in the blastoder...Continue Reading

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Citations

Mar 1, 1996·The Journal of Experimental Zoology·A T SchmidB Yedvobnick
Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J E Rooke, T Xu
Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N E Baker
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