The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management

Scientific Reports
Zhaojiang GuoYoujun Zhang

Abstract

Insect pests cause serious crop damage and develop high-level resistance to chemical insecticides and Bacillus thuringiensis (Bt) insecticidal Cry toxins. A new promising approach for controlling them and overcoming this resistance is RNA interference (RNAi). The RNAi-based insect control strategy depends on the selection of suitable target genes. In this study, we cloned and characterized a novel ABC transporter gene PxABCH1 in diamondback moth, Plutella xylostella (L.). Phylogenetic analysis showed that PxABCH1 is closely related to ABCA and ABCG subfamily members. Spatial-temporal expression detection revealed that PxABCH1 was expressed in all tissues and developmental stages, and highest expressed in head and male adult. Midgut sequence variation and expression analyses of PxABCH1 in all the susceptible and Bt-resistant P. xylostella strains and the functional analysis by sublethal RNAi demonstrated that Cry1Ac resistance was independent of this gene. Silencing of PxABCH1 by a relatively high dose of dsRNA dramatically reduced its expression and resulted in larval and pupal lethal phenotypes in both susceptible and Cry1Ac-resistant P. xylostella strains. To our knowledge, this study provides the first insight into ABCH1 in ...Continue Reading

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Citations

Jan 26, 2017·Frontiers in Pharmacology·Raafat El-AwadyGhada Elakraa
Dec 5, 2017·Entomologia Experimentalis Et Applicata·Michael DarringtonTracey Chapman
Nov 22, 2019·Neotropical Entomology·N P DiasM J Zotti
Nov 25, 2018·International Journal of Molecular Sciences·Zhaojiang GuoYoujun Zhang
Jun 13, 2019·International Journal of Molecular Sciences·Chao WuYutao Xiao
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Mar 4, 2021·Biological Reviews of the Cambridge Philosophical Society·Cheri C Choi, Robert C Ford
May 1, 2021·Animals : an Open Access Journal From MDPI·Chhavi ChoudharyJayendra Nath Shukla
Apr 20, 2021·Frontiers in Physiology·Petter F EntringerKatia C Gondim
Jul 3, 2021·International Journal of Molecular Sciences·Jianying QinZhaojiang Guo

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Datasets Mentioned

BETA
KP260785

Methods Mentioned

BETA
transgenic
RNA-seq
electrophoresis
PCR
in vitro transcription
transfection

Software Mentioned

ORF Finder
NetOGlyc
Translate
ClustalW
BLAST
MEGA
Blastn
ScanProsite
ExPASy
Molecular Evolutionary Genetic Analysis

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