The novel adipokine WISP1 associates with insulin resistance and impairs insulin action in human myotubes and mouse hepatocytes

Diabetologia
Tina HörbeltD Margriet Ouwens

Abstract

Wingless-type (Wnt) inducible signalling pathway protein-1 (WISP1) has been recently identified as a proinflammatory adipokine. We examined whether WISP1 expression and circulating levels are altered in type 2 diabetes and whether WISP1 affects insulin signalling in muscle cells and hepatocytes. Serum and visceral adipose tissue (VAT) biopsies, for analysis of circulating WISP1 levels by ELISA and WISP1 mRNA expression by real-time quantitative RT-PCR, were collected from normal-weight men (control group, n = 33) and obese men with (n = 46) and without type 2 diabetes (n = 56) undergoing surgery. Following incubation of primary human skeletal muscle cells (hSkMCs) and murine AML12 hepatocytes with WISP1 and insulin, insulin signalling was analysed by western blotting. The effect of WISP1 on insulin-stimulated glycogen synthesis and gluconeogenesis was investigated in hSkMCs and murine hepatocytes, respectively. Circulating WISP1 levels were higher in obese men (independent of diabetes status) than in normal-weight men (mean [95% CI]: 70.8 [55.2, 86.4] ng/l vs 42.6 [28.5, 56.6] ng/l, respectively; p < 0.05). VAT WISP1 expression was 1.9-fold higher in obese men vs normal-weight men (p < 0.05). Circulating WISP1 levels were posit...Continue Reading

References

Jan 10, 1998·The Journal of Biological Chemistry·Y YamanakaY Oh
Mar 31, 2000·Diabetes Research and Clinical Practice·M GuttJ B Marks
Oct 13, 2001·The Journal of Biological Chemistry·L DesnoyersD Pennica
Jul 15, 2009·Cell Cycle·Eva Gonzalez, Timothy E McGraw
Aug 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mitsuaki OnoMarian F Young
Apr 24, 2012·The Journal of Clinical Endocrinology and Metabolism·Johannes B RuigeD Margriet Ouwens
May 24, 2012·Archives of Physiology and Biochemistry·Ingrid DahlmanPeter Arner
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Oct 23, 2012·Cold Spring Harbor Perspectives in Biology·Cristina-Maria Cruciat, Christof Niehrs
Nov 16, 2012·Nature Reviews. Molecular Cell Biology·Christof Niehrs
Feb 9, 2013·The Journal of Clinical Endocrinology and Metabolism·Anna EhrlundMikael Rydén
Sep 24, 2013·Antioxidants & Redox Signaling·Magdalena KozakowskaAlicja Jozkowicz
Oct 5, 2014·Diabetes·Veronica MurahovschiNatalia Rudovich
Feb 24, 2015·Trends in Endocrinology and Metabolism : TEM·Birgit GustafsonUlf Smith
Apr 13, 2015·The Journal of Biological Chemistry·Azusa MaedaMarian F Young
May 4, 2017·Current Medicinal Chemistry·Sabine Sarah EcksteinRainer Lehmann
May 13, 2017·Scientific Reports·Nathalie FerrandMichèle Sabbah
Nov 13, 2017·Journal of Cell Communication and Signaling·Christopher TackeNatalia Rudovich
Dec 22, 2017·Journal of the Endocrine Society·Ilaria BarchettaMaria Gisella Cavallo

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Citations

Feb 27, 2019·Journal of Cellular Physiology·Habib YaribeygiAmirhossein Sahebkar
Nov 30, 2019·Journal of Cell Communication and Signaling·Vadim V KlimontovNatalia Rudovich
May 23, 2020·Bioengineering & Translational Medicine·Amrita Banerjee, Jagdish Singh
Nov 27, 2020·Nature Communications·Rebeca Fernandez-RuizRosa Gasa
Jan 28, 2021·Medicina·Małgorzata Mirr, Maciej Owecki
May 4, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Wenjing WuJin Zhang
May 1, 2021·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·Yangyang ChengJunxia Zhang

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