The novel albumin-chitosan core-shell nanoparticles for gene delivery: preparation, optimization and cell uptake investigation

Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology
Mahdi KarimiHossein Naderi-Manesh

Abstract

Natural polymers and proteins such as chitosan (CS) and albumin (Alb) have recently attracted much attention both in drug delivery and gene delivery. The underlying rationale is their unique properties such as biodegradability, biocompatibility and controlled release. This study aimed to prepare novel albumin-chitosan-DNA (Alb-CS-DNA) core-shell nanoparticles as a plasmid delivery system and find the best conditions for their preparation. Phase separation method and ionic interaction were used for preparation of Alb nanoparticles and Alb-CS-DNA core-shell nanoparticles, respectively. The effects of three important independent variables (1) CS/Alb mass ratio, (2) the ratios of moles of the amine groups of cationic polymers to those of the phosphate groups of DNA (N/P ratio), and (3) Alb concentration, on the nanoparticle size and loading efficiency of the plasmid were investigated and optimized through Box-Behnken design of response surface methodology (RSM). The optimum conditions were found to be CS/Alb mass ratio = 3, N/P ratio = 8.24 and Alb concentration = 0.1 mg/mL. The most critical factors for the size of nanoparticles and loading efficiency were Alb concentration and N/P ratio. The optimized nanoparticles had an average...Continue Reading

References

Dec 22, 1999·International Journal of Pharmaceutics·C WeberK Langer
Mar 4, 2000·International Journal of Pharmaceutics·C WeberK Langer
Feb 22, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·H Q MaoK W Leong
Aug 23, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·K A JanesM J Alonso
Apr 25, 2003·International Journal of Pharmaceutics·K LangerD Schubert
Dec 20, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anas El-Aneed
Apr 7, 2004·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sally-Ann CryanCaitriona M O'Driscoll
Mar 22, 2005·Mini Reviews in Medicinal Chemistry·J M IracheS Espuelas
Jul 9, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gottfried MayerDieter Schubert
Oct 6, 2005·Biomaterials·Sania MansouriJulio C Fernandes
Oct 26, 2005·Biomaterials·Laura De LaporteLonnie D Shea
May 23, 2006·International Journal of Pharmaceutics·Shirzad AzarmiWilson H Roa
Jun 20, 2006·Advanced Drug Delivery Reviews·Tae Gwan ParkSung Wan Kim
Jul 11, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kenneth A HowardJørgen Kjems
Oct 20, 2006·Journal of Nanoscience and Nanotechnology·Dongwon LeeShyam Mohapatra
Mar 5, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yajun DuanYaoting Yu
Jun 28, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Felix Kratz
Nov 7, 2008·Journal of Materials Science. Materials in Medicine·Ezequiel de Souza Costa-JúniorHerman S Mansur
Feb 6, 2009·Biotechnology Progress·Sufeng ZhangHasan Uludağ
May 20, 2009·BioTechniques·Raheleh RahbariRichard M Badge
Oct 3, 2009·Advanced Drug Delivery Reviews·Shirui MaoThomas Kissel
Nov 3, 2009·Biomaterials·Sufeng ZhangHasan Uludağ
Jul 27, 2010·Biophysical Journal·Kuniharu Utsuno, Hasan Uludağ
Sep 11, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Nirupam Banerjee, S B Krupanidhi
Nov 5, 2010·Chemical & Pharmaceutical Bulletin·Kalpana NagpalDina Nath Mishra
Dec 3, 2010·Journal of Nanoscience and Nanotechnology·Kuldeep K BansalNarendra K Jain
Apr 2, 2011·Journal of Nanoscience and Nanotechnology·Tae-Hun KimJoon Sig Choi
May 10, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Geetanjali AryaSanjeeb K Sahoo
Aug 16, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ahmed O ElzoghbyNazik A Elgindy
Nov 23, 2011·Journal of Nanoscience and Nanotechnology·Kyung-Man LeeIn-Joon Oh

❮ Previous
Next ❯

Citations

Jun 18, 2014·Nanoscale Research Letters·Solmaz Maleki DizajAhmad Yari Khosroushahi
Jan 21, 2015·Expert Opinion on Drug Delivery·Mahdi KarimiMichael R Hamblin
May 25, 2016·Expert Opinion on Drug Delivery·Mahdi KarimiMichael R Hamblin
Jun 29, 2016·ACS Applied Materials & Interfaces·Mahdi KarimiMichael R Hamblin
Apr 30, 2017·Nanomaterials·Michael K Riley, Wilfred Vermerris
Dec 20, 2014·Colloids and Surfaces. B, Biointerfaces·L BekaleH A Tajmir-Riahi
Mar 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Rezvan MobasseriSeeram Ramakrishna
Jan 19, 2016·Chemical Society Reviews·Mahdi KarimiMichael R Hamblin
Jun 15, 2016·Lab on a Chip·Mahdi KarimiMichael R Hamblin
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Xiao-Da LiZhi-Fei Dai
Dec 14, 2018·Environmental Science and Pollution Research International·Amr El-Sayed, Mohamed Kamel
May 3, 2019·Annals of Biomedical Engineering·Dustin J Hadley, Eduardo A Silva
Feb 6, 2020·International Journal of Veterinary Science and Medicine·Fady Sayed YoussefAhmed Mohamed Galal
Nov 18, 2018·International Journal of Molecular Sciences·Dominika Hobernik, Matthias Bros
Mar 27, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Namdev DhasTejal Mehta
Apr 5, 2016·International Journal of Biological Macromolecules·Guilherme A MagalhãesRegina C M de Paula
Jan 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Muhamad Alif RaziNoriho Kamiya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.