The novel chlamydial adhesin CPn0473 mediates the lipid raft-dependent uptake of Chlamydia pneumoniae

Cellular Microbiology
Tim FechtnerJohannes H Hegemann

Abstract

Chlamydiae are Gram-negative, obligate intracellular pathogens that pose a serious threat to public health worldwide. Chlamydial surface molecules are essential for host cell invasion. The first interaction with the host cell is thereby accomplished by the Outer membrane complex protein B (OmcB) binding to heparan sulfate moieties on the host cell surface, followed by the interaction of the chlamydial polymorphic membrane proteins (Pmps) with host cell receptors. Specifically, the interaction of the Pmp21 adhesin and invasin with its human interaction partner, the epidermal growth factor receptor, results in receptor activation, down-stream signalling and finally internalization of the bacteria. Blocking both, the OmcB and Pmp21 adhesion pathways, did not completely abolish infection, suggesting the presence of additional factors relevant for host cell invasion. Here, we show that the novel surface protein CPn0473 of Chlamydia pneumoniae contributes to the binding and invasion of infectious chlamydial particles. CPn0473 is expressed late in the infection cycle and located on the infectious chlamydial cell surface. Soluble recombinant CPn0473 as well as rCPn0473-coupled fluorescent latex beads adhere to human epithelial HEp-2 ce...Continue Reading

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Citations

Jan 30, 2018·Frontiers in Cellular and Infection Microbiology·Rafat ZrieqJohannes H Hegemann
Aug 14, 2019·Biological Chemistry·Jan N Galle, Johannes H Hegemann
May 23, 2017·Frontiers in Cellular and Infection Microbiology·Dhritiman SamantaStacey D Gilk
Oct 13, 2019·Nature Communications·Jan N GalleJohannes H Hegemann
Sep 10, 2019·Frontiers in Cellular and Infection Microbiology·Michelle B Ryndak, Suman Laal
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Corinna BraunKatja Mölleken
Aug 6, 2021·Frontiers in Microbiology·Alison Favaroni, Johannes H Hegemann

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Methods Mentioned

BETA
confocal microscopy
PCR

Software Mentioned

NIS Element
Image Pro Plus

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