The novel complex combination of alum, CpG ODN and HH2 as adjuvant in cancer vaccine effectively suppresses tumor growth in vivo

Oncotarget
Yao-Mei TianLi Yang

Abstract

Single-component adjuvant is prone to eliciting a specific type of Th1 or Th2 response. So, the development of combinatorial adjuvants inducing a robust mixed Th1/Th2 response is a promising vaccination strategy against cancer. Here, we describe a novel combination of aluminum salts (alum), CpG oligodeoxynucleotide (CpG) and innate defense regulator peptide HH2 for improving anti-tumor immune responses. The CpG-HH2 complex significantly enhanced the production of IFN-γ, TNF-α and IL-1β, promoted the uptake of antigen and strengthened the activation of p38, Erk1/2 and NF-κB in vitro, compared to CpG or HH2 alone. Immunization with NY-ESO-1 antigen plus alum-CpG-HH2 combinatorial adjuvant effectively inhibited tumor growth and reduced tumor burden in prophylactic and therapeutic tumor models and even in passive serum or cellular therapy. In addition, co-administration of NY-ESO-1 with alum-CpG-HH2 combinatorial adjuvant markedly activated NK cell cytotoxicity, induced antibody-dependent cellular cytotoxicity (ADCC), dramatically elicited cytotoxic T lymphocytes (CTLs) response, and increased infiltrating lymphocytes in tumors. Moreover, in vivo depletion of CD8+ T cells completely and depletion of NK cells partially blocked the a...Continue Reading

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Citations

Apr 29, 2020·Cancers·Anna Lucia TorneselloLuigi Buonaguro
Feb 6, 2018·Oncoimmunology·Claudia MaletzkiMichael Linnebacher
Jan 29, 2021·Vaccines·Yue ZhaoAlexandr V Bazhin
Jun 3, 2021·Viruses·Quyen-Thi Nguyen, Young-Ki Choi

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
ELISA
flow cytometry
FACS

Software Mentioned

ImageJ
SPSS

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