The novel human endogenous retrovirus-related gene, psiTPTE22-HERV, is silenced by DNA methylation in cancers

International Journal of Cancer. Journal International Du Cancer
Qiaoyi LiangShu Zheng

Abstract

The psiTPTE22 gene has been designated as a TPTE pseudogene. Our study found that the 5' part of psiTPTE22 has no sequence similarity to TPTE and contains a 3.8-kb human endogenous retrovirus (HERV) element. Because of the HERV element, the 5' part of psiTPTE22 (psiTPTE22-HERV) expresses independently as a gene. Comparison between the DNA sequences of humans and chimps indicated that psiTPTE22-HERV is human specific. We identified 3 alternatively spliced transcript variants from psiTPTE22-HERV by a PCR-based strategy, which use the transcriptional termination signal contained in the HERV element. A 402-nt ORF was contained in the 2 longer transcripts. Western blotting using antibodies produced with chemically synthesized peptide confirmed that a 15-kDa protein was translated from this ORF. RT-PCR results indicated that the ORF-containing transcripts were mainly expressed in psiTPTE22-HERV-expressing samples. Real-time quantitative RT-PCR results showed that expression of the 402-nt ORF was upregulated in normal tissues of kidney, liver, stomach, and lung but downregulated in corresponding tumor tissues. This gene is located near the centromere of chromosome 22 and has a high GC content around the promoter region. Bisulfite sequ...Continue Reading

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Citations

Jun 26, 2012·Birth Defects Research. Part A, Clinical and Molecular Teratology·Maria TzetisSophia Kitsiou-Tzeli
Sep 16, 2011·Helicobacter·Carlos ResendeAri Ristimäki
Dec 4, 2012·New Biotechnology·Jan PačesChung-Ming Chang
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May 17, 2013·Genes & Genetic Systems·Yi-Deun JungHeui-Soo Kim
May 2, 2017·Briefings in Functional Genomics·Modjtaba Emadi-BaygiParvaneh Nikpour

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