The novel human HUEL (C4orf1) protein shares homology with the DNA-binding domain of the XPA DNA repair protein and displays nuclear translocation in a cell cycle-dependent manner

The International Journal of Biochemistry & Cell Biology
D L SimVincent T K Chow

Abstract

We have previously isolated and characterized a novel human gene HUEL (C4orf1) that is ubiquitously expressed in a wide range of human fetal, adult tissues and cancer cell lines. HUEL maps to region 4p12-p13 within the short arm of chromosome 4 whose deletion is frequently associated with bladder and other carcinomas. Here we present the genomic organization, sizes and boundaries of exons and introns of HUEL. The GC-rich upstream genomic region and 5' untranslated region (UTR) together constitute a CpG island, a hallmark of housekeeping genes. The 3250 bp HUEL cDNA incorporates a 1704 bp ORF that translates into a hydrophilic protein of 568-amino acids (aa), detected as a band of approximately 70 kDa by Western blotting. We have isolated the murine homolog of HUEL which exhibits 89% nucleotide and 94% amino acid identity to its human counterpart. The HUEL protein shares significant homology with the minimal DNA-binding domain (DNA-BD) of the DNA repair protein encoded by the xeroderma pigmentosum group A (XPA) gene. Other notable features within HUEL include the putative nuclear receptor interaction motif, nuclear localization and export signals, zinc finger, leucine zipper and acidic domains. Mimosine-mediated cell cycle synch...Continue Reading

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Citations

Sep 15, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Fabrice ChimientiMichel Seve
Mar 4, 2006·The Journal of Biological Chemistry·Yong-Heng ChenMichael R Stallcup
Apr 4, 2003·Glycobiology·Christine RondaninoAnnie-Claude Roche
Mar 9, 2007·Nucleic Acids Research·Yong-Heng ChenMichael R Stallcup
Jul 1, 2005·Molecular and Cellular Biology·Yong-Heng ChenMichael R Stallcup
Jun 11, 2004·Annual Review of Nutrition·Juan P Liuzzi, Robert J Cousins
May 13, 2006·Genomics, Proteomics & Bioinformatics·Taiho KambeYuko Yamaguchi-Iwai
Dec 20, 2019·Cancer Science·Ai Hironaka-MitsuhashiTakahiro Ochiya
Apr 23, 2008·Chemical Reviews·Emily L QueChristopher J Chang

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