The novel non-mitogenic anti-CD3 antibody, mini-yCD3, delivers a partial TCR signal

International Immunopharmacology
Ming LvBeifen Shen

Abstract

Previous studies indicated that a partial T-cell receptor signal delivered by non-mitogenic anti-CD3 antibodies is critical for dampening the activated T-cell response. The mini-yCD3 is a novel non-mitogenic anti-CD3 antibody based on a murine anti-human CD3 antibody yCD3. However, the mechanism by which mini-yCD3 suppresses immune responses mediated by activated T-cells remains unknown. To elucidate its mechanism, we examined the effects of the mini-yCD3 on early signaling events in T-cells. Similar to the mitogenic anti-CD3 mAb, mini-yCD3 triggered changes in the T-cell receptor (TCR). However, unlike the mitogenic anti-CD3 stimulation, mini-yCD3 was ineffective at inducing the highly phosphorylated zeta chain and tyrosine phosphorylation of the associated tyrosine kinase ZAP-70. This proximal signaling deficiency failed to mobilize detectable Ca(2+) and translocate NF-AT into the nucleus. Additionally, the non-mitogenic anti-CD3 appeared insufficient for the redistribution of TCRs into an aggregated cap, which correlated with T-cell activation.

References

Aug 8, 1985·The New England Journal of Medicine·UNKNOWN Ortho Multicenter Transplant Study Group
Sep 1, 1994·Stem Cells·S F ZieglerM R Alderson
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·L ChatenoudJ F Bach
Jan 28, 1994·Cell·A Weiss, D R Littman
Apr 21, 1997·The Journal of Experimental Medicine·J A SmithJ A Bluestone
Oct 29, 2000·Annals of the Rheumatic Diseases·J C Morris, T A Waldmann
Aug 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sigrid HannierStéphane Demotz
Feb 6, 2003·The Journal of Clinical Investigation·Kevan C HeroldJeffrey A Bluestone
Apr 29, 2006·International Immunopharmacology·Jian LiLi Li
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Oliver DienzMercedes Rincon
Apr 21, 2007·The International Journal of Biochemistry & Cell Biology·Ming LvBeifen Shen
Aug 9, 2007·Science's STKE : Signal Transduction Knowledge Environment·Edgar SerflingAndris Avots

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