The Novel Protein Cj0371 Inhibits Chemotaxis of Campylobacter jejuni

Frontiers in Microbiology
Xueqing DuJinlin Huang

Abstract

cj0371 is a novel gene that is associated with Campylobacter jejuni virulence, and an isogenic mutant of cj0371 showed hyper chemotaxis and motility. Chemotactic motility is an important virulence factor and is involved in C. jejuni pathogenesis. Campylobacter sp. has specific variations of the common chemotaxis components, including histidine autokinase CheA, coupling scaffold protein CheV, chemotaxis response regulator protein CheY and several chemoreceptor proteins. In this study, we used immunoprecipitation combined with LC-MS/MS analyses to screen six chemotaxis pathway proteins that potentially interact with the putative protein Cj0371. qRT-PCR was used to quantitatively analyze the expression of these chemotaxis genes and basic flagella genes. The results showed that the expression of cheV, cj1110c, and cj0262c was significantly up-regulated, and four flagella genes also had up-regulated expression in the cj0371 mutant. GST pull-down analyses found that Cj0371 interacted with the receiver domain of the CheV protein. Enzyme-coupled spectrophotometric assays showed that the ATPase activity of CheA was higher when Cj0371 was not present in the chemotaxis reaction medium. Therefore, we concludes that cj0371 has a negative in...Continue Reading

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Jul 30, 2016·Frontiers in Microbiology·Xueqing DuJinlin Huang

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Citations

Apr 25, 2020·Environmental Microbiology·Miguel A MatillaTino Krell

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Methods Mentioned

BETA
co-immunoprecipitation
pull-downs
immunoprecipitation
PCR
Pull
Assay
pull-down
co-IP
motility assay

Software Mentioned

CheV

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