The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release

Molecular Brain
Teresa ObisJosep Tomas

Abstract

Protein kinase C (PKC) regulates a variety of neural functions, including neurotransmitter release. Although various PKC isoforms can be expressed at the synaptic sites and specific cell distribution may contribute to their functional diversity, little is known about the isoform-specific functions of PKCs in neuromuscular synapse. The present study is designed to examine the location of the novel isoform nPKCε at the neuromuscular junction (NMJ), their synaptic activity-related expression changes, its regulation by muscle contraction, and their possible involvement in acetylcholine release. We use immunohistochemistry and confocal microscopy to demonstrate that the novel isoform nPKCε is exclusively located in the motor nerve terminals of the adult rat NMJ. We also report that electrical stimulation of synaptic inputs to the skeletal muscle significantly increased the amount of nPKCε isoform as well as its phosphorylated form in the synaptic membrane, and muscle contraction is necessary for these nPKCε expression changes. The results also demonstrate that synaptic activity-induced muscle contraction promotes changes in presynaptic nPKCε through the brain-derived neurotrophic factor (BDNF)-mediated tyrosine kinase receptor B (Tr...Continue Reading

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Citations

Nov 4, 2015·Frontiers in Cellular Neuroscience·Jon J Brudvig, Jill M Weimer
May 26, 2017·The Journal of Biological Chemistry·Michelle DuongMario Schiffer
Jun 14, 2019·Biological Reviews of the Cambridge Philosophical Society·Zhong-Ming Qian, Ya Ke
Feb 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Víctor Cilleros-MañéMaria Angel Lanuza
May 1, 2021·International Journal of Molecular Sciences·Laia Just-BorràsMaria A Lanuza
Jun 17, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·V Cilleros-MañéM A Lanuza

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Methods Mentioned

BETA
confocal microscopy
dissection
PMA
electron microscopy
protein assay
electrophoresis

Software Mentioned

Axoscope
MARCKS
Adobe PhotoShop
MetaMorph

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