The novel therapeutic potential of bovine α-lactalbumin made lethal to tumour cells (BALMET) and oleic acid in oral squamous cell carcinoma (OSCC).

European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)
Nicoleta SineviciJeff O'Sullivan

Abstract

Since the serendipitous discovery of bovine α-lactalbumin made lethal to tumour cells (BAMLET)/human α-lactalbumin made lethal to tumour cells there has been an increased interest in the ability of the two components, oleic acid and α-lactalbumin, to form anti-cancer complexes. Here we have investigated the in-vitro efficacy of the BAMLET complex in killing oral cancer (OC) cells, determined the active component of the complex and investigated possible biological mechanisms. Two OC cell lines (±p53 mutation) and one dysplastic cell line were used as a model of progressive oral carcinogenesis. We performed cell viability assays with increasing BAMLET concentrations to determine the cytotoxic potential of the complex. We further analysed the individual components to determine their respective cytotoxicities. siRNA knockdown of p53 was used to determine its functional role in mediating sensitivity to BAMLET. Cell death mechanisms were investigated by flow cytometry, confocal microscopy and the lactate dehydrogenase assay. Our results show that BAMLET is cytotoxic to the OC and dysplastic cell lines in a time and dose-dependent manner. The cytotoxic component was found to be oleic acid, which, can induce cytotoxicity even when not ...Continue Reading

References

Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·A HåkanssonC Svanborg
Jul 1, 1996·Pathology, Research and Practice·P H Shaw
Feb 26, 1999·The Journal of Biological Chemistry·M SvenssonS Linse
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SvenssonC Svanborg
May 4, 2005·The Journal of Nutrition·Lotta GustafssonCatharina Svanborg
Feb 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·O HallgrenC Svanborg
Sep 12, 2009·Drug Metabolism and Pharmacokinetics·Ger J van der Vusse
Jan 8, 2010·Molecular Cancer Therapeutics·Paul RammerMarja Jäättelä
May 26, 2012·Biological Chemistry·Sergei E PermyakovEugene A Permyakov
Jul 24, 2012·Biochemical Society Transactions·Soyoung MinK Hun Mok
Oct 13, 2012·Frontiers in Pharmacology·Sabrina Daniela da SilvaMoulay A Alaoui-Jamali
Mar 19, 2013·Biochimica Et Biophysica Acta·Angelo FontanaPatrizia Polverino de Laureto
Dec 3, 2015·Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Société Canadienne Des Sciences Pharmaceutiques·Emma M RathWilliam Bret Church
Dec 30, 2016·JAMA Otolaryngology-- Head & Neck Surgery·Ali HosniAndrew Hope
Feb 19, 2017·Biochemical and Biophysical Research Communications·James C S HoCatharina Svanborg
Jun 24, 2017·International Journal of Dentistry·Anna Carolina Omena Vasconcellos Le CampionSonia Maria Soares Ferreira
Aug 15, 2017·Nepal Journal of Epidemiology·Neha GuptaAshish Singla

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Citations

Aug 23, 2020·Biomolecules·Eugene A Permyakov

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