The nrfI gene is essential for the attachment of the active site haem group of Wolinella succinogenes cytochrome c nitrite reductase

Molecular Microbiology
René PisaJörg Simon

Abstract

The cytochrome c nitrite reductase complex (NrfHA) is the terminal enzyme in the electron transport chain catalysing nitrite respiration of Wolinella succinogenes. The catalytic subunit NrfA is a pentahaem cytochrome c containing an active site haem group which is covalently bound via the cysteine residues of a unique CWTCK motif. The lysine residue serves as the axial ligand of the haem iron. The other four haem groups of NrfA are bound by conventional haem-binding motifs (CXXCH). The nrfHAIJ locus was restored on the genome of the W. succinogenes DeltanrfAIJ deletion mutant by integration of a plasmid, thus enabling the expression of modified alleles of nrfA and nrfI. A mutant (K134H) was constructed which contained a nrfA gene encoding a CWTCH motif instead of CWTCK. NrfA of strain K134H was found to be synthesized with five bound haem groups, as judged by matrix-assisted laser-desorption/ionization (MALDI) mass spectrometry. Its nitrite reduction activity with reduced benzyl viologen was 40% of the wild-type activity. Ammonia was formed as the only product of nitrite reduction. The mutant did not grow by nitrite respiration and its electron transport activity from formate to nitrite was 5% of that of the wild-type strain. T...Continue Reading

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Citations

Aug 8, 2002·FEMS Microbiology Reviews·Jörg Simon
Jun 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elaine R Frawley, Robert G Kranz
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·James W A AllenStuart J Ferguson
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Robert G KranzElaine R Frawley
May 6, 2010·Future Microbiology·Vincent MeuricMartine Bonnaure-Mallet
Oct 1, 2011·The FEBS Journal·Jörg Simon, Lars Hederstedt
Oct 1, 2011·The FEBS Journal·Julie M StevensStuart J Ferguson
Jul 31, 2012·Biochimica Et Biophysica Acta·Jörg Simon, Martin G Klotz
Apr 4, 2014·Chemical Reviews·Luisa B Maia, José J G Moura
Nov 14, 2019·Protein Science : a Publication of the Protein Society·Marcus J EdwardsThomas A Clarke
Nov 13, 2002·The Journal of Biological Chemistry·Patrice P HamelSabeeha Merchant
Nov 13, 2002·The Journal of Biological Chemistry·Beth Welty DreyfussSabeeha Merchant
Jan 19, 2006·Biochemical Society Transactions·S HartshorneJ Simon
Jan 24, 2020·Scientific Reports·Kenneth L BrockmanDaad A Saffarini
Dec 6, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Peter M H Kroneck

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