PMID: 9445048Jan 28, 1998Paper

The NS1 protein of human respiratory syncytial virus is a potent inhibitor of minigenome transcription and RNA replication

Journal of Virology
P L AtreyaP L Collins

Abstract

The NS1 protein (139 amino acids) is one of the two nonstructural proteins of human respiratory syncytial virus (RSV) and is encoded by a very abundant mRNA transcribed from the promoter-proximal RSV gene. The function of NS1 was unknown and was investigated here by using a reconstituted transcription and RNA replication system that involves a minireplicon and viral proteins (N, P, L and M2-1) expressed from separate cotransfected plasmids. Coexpression of the NS1 cDNA strongly inhibited transcription and RNA replication mediated by the RSV polymerase, even when the level of expressed NS1 protein was substantially below that observed in RSV-infected cells. The effect depended on synthesis of NS1 protein rather than NS1 RNA alone. Transcription and both steps of RNA replication, namely, synthesis of the antigenome and the genome, appeared to be equally sensitive to inhibition. The efficiency of encapsidation of the plasmid-derived minigenome was not altered by coexpression of NS1, indicating that the inhibition occurs at a later step. In two different dicistronic minigenomes, transcription of each gene was equally sensitive to inhibition by NS1. This suggested that the gradient of transcriptional polarity was unaffected and that...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 14, 1992·Analytical Biochemistry·P Chomczynski
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P L Collins, G W Wertz
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·P L CollinsH Grosfeld

❮ Previous
Next ❯

Citations

Apr 16, 2004·Clinical Microbiology Reviews·Andrew J EastonHelene F Rosenberg
May 2, 2007·Annals of the New York Academy of Sciences·Shobha Broor, Preeti Bharaj
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·A Bermingham, P L Collins
Mar 3, 2004·Journal of Virology·Tatjana I CornuJuan Carlos de la Torre
Apr 15, 2003·The Journal of General Virology·Gjon BlakqoriFriedemann Weber
Jan 19, 2002·Journal of Virology·Rachel FearnsPeter L Collins
Sep 3, 2011·Microbiology and Molecular Biology Reviews : MMBR·Denis Gerlier, Douglas S Lyles
Apr 19, 2008·The Journal of General Virology·Katrina SleemanPaul A Rota
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Oct 24, 2003·The Journal of General Virology·Mar PerezJuan Carlos de la Torre
Mar 22, 2002·The Journal of General Virology·R GhildyalJ Meanger
Jun 9, 2020·Frontiers in Cellular and Infection Microbiology·Elena Margaret Thornhill, David Verhoeven
Dec 6, 2007·The Journal of Infectious Diseases·Shinji WatanabeYoshihiro Kawaoka
Dec 14, 2012·PLoS Pathogens·Duncan Paterson, Ervin Fodor
Jan 30, 2007·Veterinary Research·Jean-Francois Valarcher, Geraldine Taylor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.