PMID: 9188601Jul 1, 1997Paper

The NS1 protein of the autonomous parvovirus minute virus of mice blocks cellular DNA replication: a consequence of lesions to the chromatin?

Journal of Virology
A Op De Beeck, P Caillet-Fauquet

Abstract

The nonstructural protein NS1 of the autonomous parvovirus minute virus of mice interferes with cell division and can cause cell death, depending on the cell transformation state. Upon infection, the synthesis of NS1 protein is massively initiated during S phase. In this article, we show that minute virus of mice-infected cells accumulate in this phase. To investigate the link between NS1 accumulation and S-phase arrest, we have used stably transfected cells in which NS1 expression is under the control of a glucocorticoid-inducible promoter (the long terminal repeat of mouse mammary tumor virus). NS1 expression interferes with cell DNA replication, and consequently, the cell cycle stops in S phase. NS1 expression also induces nicks in the cell chromatin, as detected by an in situ nick translation assay. The nicks are observed several hours before any cell cycle perturbation. As cell cycle arrest is a common consequence of DNA damage, we propose that NS1 exerts its cytostatic activity by inducing lesions in cell chromatin.

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M JakóbisiakZ Darzynkiewicz
Jan 1, 1987·Advances in Virus Research·S F Cotmore, P Tattersall
Dec 1, 1995·Journal of Virology·H ZhuT Shenk
Feb 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W K Kaufmann, R S Paules
Sep 1, 1996·Current Biology : CB·M Emerman
Mar 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·H M SHEIN, J F ENDERS

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Citations

Oct 17, 2001·Journal of Virology·A Op De BeeckP Caillet-Fauquet
May 21, 2005·Chinese Journal of Digestive Diseases·Jiong LiuJean Rommelaere
Feb 1, 2013·Journal of Virology·Yong LuoJianming Qiu
Jan 15, 2014·PLoS Pathogens·Richard O Adeyemi, David J Pintel
Mar 1, 2013·Future Virology·Yong Luo, Jianming Qiu
Oct 30, 2004·The Journal of Gene Medicine·Karim El BakkouriAnnick Brandenburger
Apr 28, 2006·Journal of Virology·Jürg P F Nüesch, Jean Rommelaere
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carole BerthetPeter Beard
Jun 10, 2011·Memórias do Instituto Oswaldo Cruz·Ana Luiza Pamplona MosimannClaudia Nunes Duarte Dos Santos
Dec 7, 2007·BJOG : an International Journal of Obstetrics and Gynaecology·T R de HaanF J Walther
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Feb 19, 2011·Future Virology·Aaron Yun Chen, Jianming Qiu
Jan 30, 2015·Virology Journal·Antonio MarchiniJean Rommelaere
Jul 1, 2020·Annual Review of Virology·Anna HartleyAntonio Marchini
Jun 27, 2014·Journal of Virology·Richard O Adeyemi, David J Pintel
Apr 3, 1998·Journal of Virology·L J Ball-GoodrichR O Jacoby
Dec 5, 2018·Frontiers in Cellular and Infection Microbiology·Ying FanRoberto Bruzzone
Oct 5, 2017·Viruses·Elina MäntyläMaija Vihinen-Ranta
Oct 6, 2000·Medical Hypotheses·F F Shadan, L P Villarreal
Nov 3, 2004·Virology·Akitoshi NakashimaKazuo Sugamura
Aug 11, 2021·Viruses·Salla MattolaMaija Vihinen-Ranta

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