The NS2 polypeptide of parvovirus MVM is required for capsid assembly in murine cells

Virology
Susan F CotmorePeter Tattersall

Abstract

Mutants of minute virus of mice (MVM) which express truncated forms of the NS2 polypeptide are known to exhibit a host range defect, replicating productively in transformed human cells but not in cells from their normal murine host. To explore this deficiency we generated viruses with translation termination codons at various positions in the second exon of NS2. In human cells these mutants were viable, but showed a late defect in progeny virion release which put them at a selective disadvantage compared to the wildtype. In murine cells, however, duplex viral DNA amplification was reduced to 5% of wildtype levels and single-strand DNA synthesis was undetectable. These deficiencies could not be attributed to a failure to initiate infection or to a generalized defect in viral gene expression, since the viral replicator protein NS1 was expressed to normal or elevated levels early in infection. In contrast, truncated NS2 gene products failed to accumulate, so that each mutant exhibited a similar NS2-null phenotype. Expression of the capsid polypeptides VP1 and VP2 and their subsequent assembly into intact particles were examined in detail. Synchronized infected cell populations labeled under pulse-chase conditions were analyzed by ...Continue Reading

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Citations

Sep 5, 2008·Journal of Virology·Karin HoelzerColin R Parrish
May 22, 2002·Journal of Virology·Philip J YoungChristian L Lorson
Sep 20, 2002·Journal of Virology·Marty R BadgettJames J Bull
Dec 13, 2002·Journal of Virology·Jürg P F NüeschJean Rommelaere
Jan 19, 1999·Annual Review of Microbiology·H Kasamatsu, A Nakanishi
Jan 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Laura A ShackeltonEdward C Holmes
Sep 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·Muhammed P SafeenaIndrani Karunasagar
Mar 26, 2010·Archives of Virology·Pongsopee AttasartSakol Panyim
Feb 17, 2010·Virus Research·Muhammed P SafeenaIndrani Karunasagar
Mar 10, 2016·Annual Review of Virology·Shweta KailasanColin R Parrish
Mar 21, 2001·The Journal of General Virology·M Söderlund-VenermoD J Pintel
Jun 15, 2011·Biochimica Et Biophysica Acta·Jiwon HwangRobert F Kalejta
Sep 25, 1999·Acta Veterinaria Hungarica·P Tijssen
Oct 21, 1999·Biochemical and Biophysical Research Communications·T OhshimaK i Yagami
Aug 8, 2008·Virology·Igor EtingovClaytus Davis

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