The nsp1alpha and nsp1 papain-like autoproteinases are essential for porcine reproductive and respiratory syndrome virus RNA synthesis

The Journal of General Virology
Michiel V KroeseEric J Snijder

Abstract

The two N-terminal cleavage products, nsp1alpha and nsp1beta, of the replicase polyproteins of porcine reproductive and respiratory syndrome virus (PRRSV) each contain a papain-like autoproteinase domain, which have been named PCPalpha and PCPbeta, respectively. To assess their role in the PRRSV life cycle, substitutions and deletions of the presumed catalytic cysteine and histidine residues of PCPalpha and PCPbeta were introduced into a PRRSV infectious cDNA clone. Mutations that inactivated PCPalpha activity completely blocked subgenomic mRNA synthesis, but did not affect genome replication. In contrast, mutants in which PCPbeta activity was blocked proved to be non-viable and no sign of viral RNA synthesis could be detected, indicating that the correct processing of the nsp1beta/nsp2 cleavage site is essential for PRRSV genome replication. In conclusion, the data presented here show that a productive PRRSV life cycle depends on the correct processing of both the nsp1alpha/nsp1beta and nsp1beta/nsp2 junctions.

References

Sep 1, 1994·Journal of Virology·E J SnijderW J Spaan
Mar 22, 2000·The Journal of General Virology·J ZiebuhrA E Gorbalenya
Mar 22, 2002·Journal of Virology·Rainer GosertSusan C Baker
Mar 1, 2006·Virus Research·Alexander E GorbalenyaEric J Snijder
May 13, 2006·The Journal of General Virology·Alexander O PasternakEric J Snijder

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Citations

Jan 5, 2014·The Journal of Microbiology·Sang-Im Yun, Young-Min Lee
Nov 26, 2010·Virology Journal·Roongtham KedkovidRoongroje Thanawongnuwech
May 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yanhua LiYing Fang
Aug 14, 2013·The Journal of General Virology·Eric J SnijderYing Fang
Dec 3, 2014·Virus Research·Margo A BrintonHeather A Vatter
Dec 3, 2014·Veterinary Microbiology·Mingyuan Han, Dongwan Yoo
Mar 28, 2017·Veterinary Microbiology·Hanzhong Ke, Dongwan Yoo
Dec 24, 2019·The Journal of Veterinary Medical Science·Fujiko SunagaTetsuya Mizutani
Oct 22, 2019·Emerging Microbes & Infections·Tong-Yun WangXue-Hui Cai

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