The NtrY/X two-component system of Brucella spp. acts as a redox sensor and regulates the expression of nitrogen respiration enzymes

Molecular Microbiology
Mariela Del Carmen CarricaFernando Alberto Goldbaum

Abstract

Brucella spp. are facultative intracellular bacteria pathogenic for many mammalian species including humans, causing a disease called brucellosis. Learning how Brucella adapts to its intracellular niche is crucial for understanding its pathogenesis mechanism, allowing for the development of new and more effective vaccines and treatments against brucellosis. Brucella pathogenesis resides mostly in its ability to adapt to the harsh environmental conditions encountered during host infection such as the oxygen depletion. The mechanism by which Brucella senses the oxygen tension and triggers its environmental adaptation is unknown. In this work we show that the Brucella abortus NtrY/NtrX two-component system is involved in oxygen sensing through a haem group contained in a Per-ARNT-SIM (PAS) domain of the NtrY histidine kinase. The NtrY haem iron can be reduced to the ferrous form and is rapidly oxidized to the ferric form in presence of oxygen. Importantly, we show that the oxidation state of the haem iron modulates the autokinase activity, being the anoxygenic reduced ferrous form the signalling state of NtrY. Also, we show that ntrY gene expression increases under low oxygen tension and that NtrY transfers its signal to its cogna...Continue Reading

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Citations

Dec 5, 2014·Critical Reviews in Microbiology·Severin RonneauJean-Jacques Letesson
May 16, 2012·Molecular Microbiology·R Martin Roop, Clayton C Caswell
Mar 27, 2013·Molecular Microbiology·Mariela Del Carmen CarricaFernando Alberto Goldbaum
Jun 27, 2015·Journal of Molecular Biology·Ignacio FernándezFernando A Goldbaum
May 26, 2017·MBio·Kimberly C LemmerTimothy J Donohue
Apr 11, 2018·PloS One·Ignacio FernándezMariela Del Carmen Carrica
Jul 12, 2017·Journal of Veterinary Science·Jun-Gu KangJoon-Seok Chae
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Jun 9, 2020·Acta Biochimica Et Biophysica Sinica·Shuang ZengLi Luo
Nov 22, 2019·Chemical Society Reviews·Toru ShimizuMarkéta Martínková
Feb 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·M Ashley BoneSteven M Julio
Nov 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·J Elijah PowellNancy A Moran
Apr 11, 2018·Frontiers in Microbiology·Ruiping ZhengZhihui Cheng
May 21, 2020·MBio·Kimberly C LemmerTimothy J Donohue
Feb 12, 2021·Microbiology and Molecular Biology Reviews : MMBR·R Martin RoopDaniel W Martin

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