The nuclear GSK-3β regulated post-transcriptional processing of mRNA through phosphorylation of SC35

Molecular and Cellular Biochemistry
Yu AnBo Zhang

Abstract

Glycogen synthase kinase-3β (GSK-3β) is a multifunctional serine/threonine kinase and regulates a variety of biological processes. Recent studies show GSK-3β can regulate pre-mRNA processing and transcription through phosphorylation of multiple splicing factors, but the detailed mechanism is still undetermined. In this study, we further proved that GSK-3β could specifically co-localize with SC35 in nuclear speckles depending on its kinase activity. Immunofluorescence and FISH studies showed the activity of nuclear GSK-3β regulated the assembly of nuclear speckles and consequently modulated the post-transcriptional processing of mRNA. In addition, GSK-3β phosphorylated SC35 and promoted its hyperphosphorylation, in which the unique C-terminal sequences were particularly important to efficiently sequential multiple phosphorylation of SC35. Hyperphosphorylated SC35 converged into cluster and lost its ability to perform splicing in nuclear speckles. More importantly, the nuclear GSK-3β activity could be a part of Wnt/β-catenin signaling activation by TCF4 and might take part in embryonic or tumorigenesis of cells.

References

May 1, 1992·Genes & Development·A M ZahlerM B Roth
Jan 1, 1983·Biology of the Cell·D L SpectorH Busch
Feb 1, 1994·The Journal of Cell Biology·R T O'KeefeD L Spector
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·M HoshiK Imahori
Aug 10, 2001·The Journal of Biological Chemistry·G N Bijur, R S Jope
Aug 31, 2001·Progress in Neurobiology·C A Grimes, R S Jope
Sep 7, 2002·Molecular and Cellular Biology·Demian CazallaJavier F Cáceres
Mar 13, 2003·Molecular Biology of the Cell·Kannanganattu V PrasanthDavid L Spector
Aug 19, 2003·Nature Reviews. Molecular Cell Biology·Angus I Lamond, David L Spector
Apr 23, 2004·Trends in Biochemical Sciences·Richard S Jope, Gail V W Johnson
Aug 19, 2007·BMC Cell Biology·Anthony TigheStephen S Taylor
Jul 22, 2008·Nature Structural & Molecular Biology·Shengrong LinXiang-Dong Fu
Dec 9, 2008·The Biochemical Journal·Jennifer C Long, Javier F Caceres
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Jul 10, 2010·Neurobiology of Disease·Kun-Lin ChenChung Y Hsu
Oct 12, 2010·Cold Spring Harbor Perspectives in Biology·David L Spector, Angus I Lamond
Jan 6, 2011·The FEBS Journal·Gourisankar Ghosh, Joseph A Adams
Apr 8, 2011·Nucleic Acids Research·Wei QianFei Liu
Jun 2, 2011·International Journal of Alzheimer's Disease·Miguel Medina, Francisco Wandosell
Jul 29, 2011·The FEBS Journal·Laure TwyffelsVéronique Kruys
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Apr 23, 2013·Cell Reports·Markku VarjosaloMatthias Gstaiger
Sep 13, 2013·Cellular and Molecular Life Sciences : CMLS·Dietmar RiederZlatko Trajanoski
Aug 27, 2014·Wiley Interdisciplinary Reviews. RNA·Jonathan M Howard, Jeremy R Sanford
Dec 2, 2014·Pharmacology & Therapeutics·Eleonore BeurelRichard S Jope
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jian ZhangJames L Manley
Nov 26, 2015·IUBMB Life·Nydia Tejeda-Muñoz, Martha Robles-Flores
Jun 11, 2017·The Journal of Cell Biology·Marie-Louise Hammarskjold, David Rekosh
Oct 5, 2017·Nucleic Acids Research·Lukasz GalganskiWlodzimierz J Krzyzosiak

❮ Previous
Next ❯

Citations

Dec 31, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Camilla EvangelistiAlberto M Martelli

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
co-immunoprecipitation
immunoprecipitation
ubiquitination
acetylation

Software Mentioned

Works
Lab
Photoshop
SPSS

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.