The nuclear receptor REV-ERBα represses the transcription of growth/differentiation factor 10 and 15 genes in rat endometrium stromal cells

Physiological Reports
Lijia ZhaoMasa-aki Hattori

Abstract

Cellular oscillators in the uterus play critical roles in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes, including growth/differentiation factor (Gdf)10 and Gdf15. The expression of Gdf10 and Gdf15 is significantly increased in the uterus during decidualization, but the mechanism underlying the regulation of Gdf gene expression in the uterus is poorly understood. Here, we focused on the function of the cellular oscillators in the expression of Gdf family by using uterine endometrial stromal cells (UESCs) isolated from pregnant Per2-dLuc transgenic rats. A significant decline of Per2-dLuc bioluminescence activity was induced in in vitro decidualized UESCs, and concomitantly the expression of canonical clock genes was downregulated. Conversely, the expression of Gdf10 and Gdf15 of the Gdf was upregulated. In UESCs transfected with Bmal1-specific siRNA, in which Rev-erbα expression was downregulated, Gdf10 and Gdf15 were upregulated. However, Gdf5, Gdf7, and Gdf11 were not significantly affected by Bmal1 silencing. The expression of Gdf10 and Gdf15 was enhanced after treatment with a REV-ERBα antagonist in the presence or absence of progesterone. Chromatin immunoprecipitat...Continue Reading

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Citations

Apr 25, 2019·Chronobiology International·Meng-Jun QiuSheng-Li Yang
Jun 9, 2020·Journal of Enzyme Inhibition and Medicinal Chemistry·Shenzhen HuangZhijie Li
Jan 8, 2020·International Journal of Molecular Sciences·Luiz Gustavo de Almeida ChuffaFábio Rodrigues Ferreira Seiva
Feb 6, 2020·International Journal of Molecular Sciences·Piotr KaczynskiAgnieszka Waclawik
Nov 6, 2020·Journal of Toxicology and Environmental Health. Part a·Lijia ZhaoHuatao Chen
Jan 20, 2021·Cellular and Molecular Life Sciences : CMLS·Susanne KlausMario Ost
Feb 9, 2021·American Journal of Physiology. Endocrinology and Metabolism·Lijia ZhaoHuatao Chen

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Methods Mentioned

BETA
transgenic
electrophoresis
transfection
PCR
immunoprecipitation
ChIP

Software Mentioned

SigmaPlot
Timing Series Single
Agilent Future Extraction

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