The nucleic acid positive rate and genotype distribution of human cytomegalovirus in human milk banks in China

Archives of Virology
Xiaoyu MinWenbo Xu

Abstract

To determine the status of human cytomegalovirus (HCMV) infection in human milk in China, a total of 510 human milk samples obtained from three provinces, including 211 donor human milk samples from human milk banks and 299 milk samples obtained from the mothers of premature infants, were tested to detect HCMV DNA. Overall, 46.4% of the donated milk samples and 59.2% of the samples obtained from mothers of premature infants were positive for HCMV DNA. The concentration of HCMV DNA was approximately 103 -104 copies/ml in the HCMV-DNA-positive human milk samples. Based on the nucleotide sequence of a 299- to 305-bp fragment of the glycoprotein B (gB) gene, three HCMV genotypes (gB1, gB2 and gB3) were identified in human milk samples. Mixed infection with genotypes gB1 and gB3 was also found in four milk samples from mothers. Genotype gB1 was the predominant genotype in the HCMV-DNA-positive human milk samples, and it could be subdivided into three lineages. There were also some characteristic nucleotides and amino acids in the three HCMV genotypes, which were helpful for distinguishing the genotypes. This is the first study to clarify the HCMV infection status and genetic characteristics of human milk obtained from banks in China...Continue Reading

References

Mar 5, 1992·The New England Journal of Medicine·K B FowlerC A Alford
May 21, 1998·The Journal of Infectious Diseases·U Meyer-KönigF T Hufert
May 29, 1998·Research in Virology·D H SheppR G Pergolizzi
Jul 18, 2000·Journal of Medical Virology·D E TrincadoW D Rawlinson
Nov 17, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J MaschmannC P Speer
Dec 18, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Lisa SarcinellaAtul Humar
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alain CoaquetteGeorges Herbein
Mar 8, 2005·Journal of Clinical Microbiology·Johannes MeierSusanna Prösch
Nov 10, 2005·Early Human Development·Klaus HamprechtJens Maschmann
Sep 25, 2009·Clinical and Vaccine Immunology : CVI·Feng-Qin FangYu-Hua Ji
Dec 23, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Xiaojin WuYuejun Liu
Jun 22, 2010·Reviews in Medical Virology·Michael J CannonTerri B Hyde
Apr 16, 2011·Canadian Journal of Microbiology·Massimiliano BergalloRossana Cavallo
Jul 1, 2010·Nutrients·Willemijn E CorpeleijnJohannes B van Goudoever
Jan 9, 2013·Clinical Microbiology Reviews·Sheetal ManicklalRavindra K Gupta
Jul 12, 2013·BMC Infectious Diseases·Débora C DieamantAfonso C Vigorito
Oct 29, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M RycelD Nowakowska
Dec 3, 2014·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Na TangFang He
Jan 3, 2015·Pediatrics·Stacy J CarlingChristine M Olson
Jun 17, 2016·Journal of Perinatology : Official Journal of the California Perinatal Association·C CorreaC Limia
Oct 7, 2016·Journal of Korean Medical Science·Hye Lim JangSung Hoon Chung
Feb 8, 2018·Acta Paediatrica·J C PicaudUNKNOWN Working group of the French Neonatal Society on fresh human milk use in preterm infants
Feb 28, 2018·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Soya S SamColleen S Kraft
Feb 24, 2019·World Journal of Pediatrics : WJP·Xi-Hong LiuUNKNOWN Group of Child Health Care, Society of Pediatrics, Chinese Medical Association

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
GU552457
MN160151-MN160207

Methods Mentioned

BETA
PCR
nucleic
genotyping

Software Mentioned

MAGE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.