The nucleolar phosphoprotein B23 interacts with hepatitis delta antigens and modulates the hepatitis delta virus RNA replication

The Journal of Biological Chemistry
W H HuangY H Lee

Abstract

Hepatitis delta virus (HDV) encodes two isoforms of delta antigens (HDAgs). The small form of HDAg is required for HDV RNA replication, while the large form of HDAg inhibits the viral replication and is required for virion assembly. In this study, we found that the expression of B23, a nucleolar phosphoprotein involved in disparate functions including nuclear transport, cellular proliferation, and ribosome biogenesis, is up-regulated by these two HDAgs. Using in vivo and in vitro experimental approaches, we have demonstrated that both isoforms of HDAg can interact with B23 and their interaction domains were identified as the NH(2)-terminal fragment of each molecule encompassing the nuclear localization signal but not the coiled-coil region of HDAg. Sucrose gradient centrifugation analysis indicated that the majority of small HDAg, but a lesser amount of the large HDAg, co-sedimented with B23 and nucleolin in the large nuclear complex. Transient transfection experiments also indicated that introducing exogenous full-length B23, but not a mutated B23 defective in HDAg binding, enhanced HDV RNA replication. All together, our results reveal that HDAg has two distinct effects on nucleolar B23, up-regulation of its gene expression an...Continue Reading

References

Apr 1, 1992·Journal of Virology·W S RyuJ Taylor
Jun 1, 1990·The Journal of General Virology·T B MacnaughtonC J Burrell
Jan 1, 1992·The Journal of Cell Biology·B A Peculis, J G Gall
Feb 1, 1991·Trends in Biochemical Sciences·B Sollner-Webb, E B Mougey
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F L ChangD S Chen
May 1, 1991·Journal of Virology·J S Glenn, J M White
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P J ChenD S Chen
Oct 23, 1990·Biochimica Et Biophysica Acta·N FeuersteinW T Ruyechan
Nov 28, 1989·Biochemistry·T S DumbarM O Olson
Mar 3, 1989·JAMA : the Journal of the American Medical Association·J H Hoofnagle
Oct 8, 1987·Nature·G Jordan
Aug 15, 1988·European Journal of Biochemistry·M S ErardF Amalric
Jul 16, 1987·Biochimica Et Biophysica Acta·B Y Yung, P K Chan
Sep 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Rizzetto
Oct 11, 1995·Nucleic Acids Research·J E HerreraM O Olson
Jan 1, 1995·Annual Review of Biochemistry·M M Lai
Jun 1, 1995·Current Opinion in Cell Biology·T Mélèse, Z Xue
Jan 1, 1993·Journal of Virology·J G Wang, S M Lemon

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Mar 27, 2004·Developmental and Comparative Immunology·Daniel R BarredaMiodrag Belosevic
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Jun 16, 2006·Cell Research·Szecheng J LoHuey-Jen Lai
Aug 19, 2005·Molecular and Cellular Biology·V SwaminathanTapas K Kundu
Jan 15, 2014·Cell Death and Differentiation·S A H de Poot, N Bovenschen
Apr 29, 2009·Reviews in Medical Virology·Anna Greco
Sep 8, 2006·The EMBO Journal·María A GarcíaCarmen Rivas
Jun 30, 2011·Reviews in Medical Virology·Robert van Domselaar, Niels Bovenschen
Jun 12, 2010·Immunological Reviews·Inna S AfoninaSeamus J Martin
Feb 5, 2008·Biochemical and Biophysical Research Communications·Yingchun ZengZhenghui Wu
May 14, 2011·The Biochemical Journal·Stefanie A H de PootNiels Bovenschen
Dec 15, 2010·Biochemistry Research International·Mikael S Lindström
Jun 19, 2013·Journal of Proteomics·Marta MendesCelso Cunha
Dec 1, 2009·Viruses·Chung-Hsin Tseng, Michael M C Lai
Jan 1, 2010·Viruses·Valerie Greco-Stewart, Martin Pelchat
Mar 29, 2018·Infectious Disorders Drug Targets·Yadira Lobaina, Yasser Perera
Sep 19, 2009·Expert Review of Anticancer Therapy·Natalia Meani, Myriam Alcalay
May 6, 2005·American Journal of Physiology. Cell Physiology·Tongtong ZouJian-Ying Wang
Oct 29, 2002·The Journal of Biological Chemistry·Shiow-Yi ChenYan-Hwa Wu Lee

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