The nucleotide and deduced amino acid sequences of EcoRI fragment containing the 5'-terminal region of Clostridium botulinum type E toxin gene cloned from Mashike, Iwanai and Otaru strains

Microbiology and Immunology
N FujiiK Oguma

Abstract

Chromosomal DNAs were extracted from toxigenic three Clostridium botulinum type E strains isolated from food-borne botulism. After digestion by EcoRI, the fragments were cloned into Escherichia coli by using bacteriophage lambda gt11 and screened with monoclonal antibody recognizing the light chain component of botulinum type E toxin. The fragments (about 1 kbp size) cloned from each strain were recloned into a plasmid vector pUC118. The E. coli cells transformed with the recombinant plasmids produced 33 kDa protein with or without IPTG (isopropyl-beta-D-thiogalactopyranoside) which reacted with the monoclonal antibody. The nucleotide sequences of the cloned EcoRI fragments from the three type E strains were identical and contain the 5'-terminal region of the type E toxin gene. It was also found that there exist several highly homologous nucleotide sequences among the botulinum types A, C and E, and tetanus toxin genes in both translated and untranslated regions.

References

Sep 28, 1990·Biochemical and Biophysical Research Communications·K KimuraK Oguma
Sep 1, 1989·Infection and Immunity·K MoriishiK Oguma
Aug 15, 1989·Biochemical and Biophysical Research Communications·M J BetleyB R DasGupta
Jan 1, 1988·Applied and Environmental Microbiology·N FujiiK Tsuzuki

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Citations

Nov 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·N FujiiK Oguma
Oct 31, 2000·Applied and Environmental Microbiology·X WangS Nakamura
Sep 1, 1993·Applied and Environmental Microbiology·E A SzaboP M Desmarchelier
Mar 1, 1992·Microbiological Reviews·E J Schantz, E A Johnson
Aug 13, 2005·Systematic and Applied Microbiology·Aparna DixitLokendra Singh

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