The nucleotide excision repair (NER) system of Helicobacter pylori: role in mutation prevention and chromosomal import patterns after natural transformation.

BMC Microbiology
Claudia MocciaSebastian Suerbaum

Abstract

Extensive genetic diversity and rapid allelic diversification are characteristics of the human gastric pathogen Helicobacter pylori, and are believed to contribute to its ability to cause chronic infections. Both a high mutation rate and frequent imports of short fragments of exogenous DNA during mixed infections play important roles in generating this allelic diversity. In this study, we used a genetic approach to investigate the roles of nucleotide excision repair (NER) pathway components in H. pylori mutation and recombination. Inactivation of any of the four uvr genes strongly increased the susceptibility of H. pylori to DNA damage by ultraviolet light. Inactivation of uvrA and uvrB significantly decreased mutation frequencies whereas only the uvrA deficient mutant exhibited a significant decrease of the recombination frequency after natural transformation. A uvrC mutant did not show significant changes in mutation or recombination rates; however, inactivation of uvrC promoted the incorporation of significantly longer fragments of donor DNA (2.2-fold increase) into the recipient chromosome. A deletion of uvrD induced a hyper-recombinational phenotype. Our data suggest that the NER system has multiple functions in the geneti...Continue Reading

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Citations

Dec 5, 2013·Nucleic Acids Research·Juliane KrebesSebastian Suerbaum
Sep 15, 2014·International Journal of Medical Microbiology : IJMM·Juliane KrebesSebastian Suerbaum
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xavier DidelotSebastian Suerbaum
Jun 23, 2016·Nature Communications·Sebastian BubendorferSebastian Suerbaum
Jan 28, 2017·Scientific Reports·Christopher CorbinaisStéphanie Marsin
Oct 14, 2020·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Yi-Bo HuangZhao Han
Sep 4, 2020·FEMS Microbiology Reviews·Florent AilloudSebastian Suerbaum

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Methods Mentioned

BETA
gene knock-out
PCR
gene knock-outs

Software Mentioned

Bionumerics
CLC Sequence Viewer

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