PMID: 377014Mar 27, 1979Paper

The nucleotide sequence of a DNA fragment from the replication origin of the antibiotic resistance factor R1drd19

Molecular & General Genetics : MGG
W OertelW Goebel

Abstract

The recombinant plasmid pRK101 contains a DNA fragment which carries the complete replication origin of the antibiotic resistance factor R1drd-19 inserted into the vector plasmid pBR322. In a spontaneously arising mutant of this plasmid (pRK103) a deletion of about 215 base pairs (bp) has been detected by heteroduplex analysis and mapping with restriction endonucleases. Essential parts of the replication origin must be located in the deleted sequence. The deletion mutant pRK103, in contrast to its parent plasmid pRK101 is not replicated under the control of the R1 replicon, even when the R1 factor or copy mutants of it are present within the same cell. These latter plasmids can complement a plasmid-specific protein not coded by pRK101 but essential for R1-directed replication. The nucleotide sequence of a 252 bp HpaII fragment covering about 170--200 bp of the deletion was determined. This piece of DNA is rich in G and C and contains a series of small palindromes, symmetrically arranged repeated sequences and short selfcomplementary structures which may be of significance for the initiation of the DNA replication. The possiblity that the sequenced DNA fragment comprises a major part of the replication origin of R1drd-19 is disc...Continue Reading

References

Oct 20, 1977·Nature·B Alberts, R Sternglanz
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Citations

Feb 1, 1980·Molecular & General Genetics : MGG·R KollekW Goebel
Jan 1, 1984·Molecular & General Genetics : MGG·T MurotsuK Matsubara
Apr 1, 1987·Molecular & General Genetics : MGG·S Iordanescu
Jan 1, 1981·Molecular & General Genetics : MGG·D M StalkerD R Helinski
Jan 1, 1981·Molecular & General Genetics : MGG·P StougaardF G Hansen
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S A KhanR P Novick
Jun 1, 1982·Journal of Bacteriology·R Diaz, W L Staudenbauer
Sep 1, 1988·Microbiological Reviews·M CouturierW K Maas
Mar 1, 1984·Microbiological Reviews·J R Scott
Jul 25, 1982·Journal of Molecular Biology·M E Brawner, S R Jaskunas

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