PMID: 2994007Aug 12, 1985

The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonas transposon Tn501

Nucleic Acids Research
N L BrownR D Pridmore


The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr = 111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR gene. The amino acid sequence predicted from this reading frame shows 32% identity with that of the transposase of the related transposon Tn3. The C-terminal regions of these two polypeptides show slightly greater homology than the N-terminal regions when conservative amino acid substitutions are considered. With this sequence determination, the nucleotide sequence of Tn501 is fully defined. The main features of the sequence are briefly presented.


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Oct 11, 1988·Nucleic Acids Research·S E West, B H Iglewski
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Nov 1, 1990·Plasmid·J GrinstedR Schmitt
May 14, 2011·FEMS Microbiology Reviews·Sally R Partridge
Apr 16, 1998·Journal of Bacteriology·T J MerkelS Stibitz


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Jan 1, 1984·Annual Review of Biochemistry·P H von HippelJ A McSwiggen
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May 10, 1980·Nucleic Acids Research·N L BrownP R Whitehead
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P A KittsD J Sherratt
Jan 1, 1981·Annual Review of Genetics·N Kleckner
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Jan 1, 1983·Molecular & General Genetics : MGG·W P DiverR Schmitt
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Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
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Related Concepts

Transcription, Genetic
Sense Codon
Pseudomonas aeruginosa
Genes, Bacterial
DNA, Bacterial
DNA Transposons

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