PMID: 2994007Aug 12, 1985

The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonas transposon Tn501

Nucleic Acids Research
N L BrownR D Pridmore

Abstract

The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr = 111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR gene. The amino acid sequence predicted from this reading frame shows 32% identity with that of the transposase of the related transposon Tn3. The C-terminal regions of these two polypeptides show slightly greater homology than the N-terminal regions when conservative amino acid substitutions are considered. With this sequence determination, the nucleotide sequence of Tn501 is fully defined. The main features of the sequence are briefly presented.

References

Jan 1, 1993·Molecular & General Genetics : MGG·T MaekawaE Ohtsubo
Feb 1, 1989·Molecular & General Genetics : MGG·C ReimmannD Haas
Feb 25, 1987·Nucleic Acids Research·E Ward, J Grinsted
Oct 11, 1988·Nucleic Acids Research·S E West, B H Iglewski
Sep 12, 2007·Antimicrobial Agents and Chemotherapy·Sung-Pin TsengLee-Jene Teng
May 1, 1989·Antimicrobial Agents and Chemotherapy·T Yamamoto
Nov 1, 1990·Plasmid·J GrinstedR Schmitt
May 14, 2011·FEMS Microbiology Reviews·Sally R Partridge
Apr 16, 1998·Journal of Bacteriology·T J MerkelS Stibitz

Citations

Oct 1, 1979·Molecular & General Genetics : MGG·A Arthur, D J Sherratt
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T K MisraS Silver
Jan 1, 1984·Molecular & General Genetics : MGG·J Grinsted, N L Brown
Jan 1, 1984·Annual Review of Biochemistry·P H von HippelJ A McSwiggen
Jan 1, 1984·Annual Review of Biochemistry·C O Pabo, R T Sauer
May 10, 1980·Nucleic Acids Research·N L BrownP R Whitehead
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P A KittsD J Sherratt
Jan 1, 1981·Annual Review of Genetics·N Kleckner
Jan 1, 1983·Molecular & General Genetics : MGG·J Altenbuchner, R Schmitt
Jan 1, 1983·Molecular & General Genetics : MGG·W P DiverR Schmitt
Jan 1, 1984·Molecular & General Genetics : MGG·P Rogowsky, R Schmitt
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Jan 11, 1984·Nucleic Acids Research·R Staden

Related Concepts

Transposase
Transcription, Genetic
Sense Codon
Pseudomonas aeruginosa
Genes, Bacterial
DNA, Bacterial
DNA Transposons
Nucleotidyltransferase

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