PMID: 7371641Apr 1, 1980Paper

The nucleotide sequences of the 5'-terminal T1 oligonucleotides of Semliki-Forest-virus 42-S and 26-S RNAs are different

European Journal of Biochemistry
R F PetterssonL Kääriäinen

Abstract

To study the mechanism of the synthesis of Semliki Forest virus (SFV) 26-S RNA, we have isolated the 5'-terminal 'cap'-containing RNase-T1-resistant oligonucleotide (T1 cap) from the genomic 42-S RNA and from the subgenomic 26-S RNA and determined their nucleotide sequences. The T1 caps were purified from 32P-labelled RNAs on two successive two-dimensional fractionation systems: (a) electrophoresis on cellulose acetate paper followed by homochromatography and (b) two-dimensional polyacrylamide gel electrophoresis. The T1 caps derived from the two RNAs had different mobilities in both systems. Their nucleotide sequence was found to be: m7G(5')ppp-(5')ApUpGp- for the 42-S RNA and m7G(5')ppp(5')ApUpUpGp- for the 26-S RNA, respectively. Thus, it appears that the 26-S RNA is not formed by initiation of the RNA polymerase at the 3' end of the negative-strand template followed by 'cleavage and splicing' or as the result of a 'polymerase jump'. Our results, instead, favour the model of internal initiation of the polymerase on the 42-S negative-strand RNA template.

References

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Citations

May 20, 1999·Antiviral Research·A LampioL Kääriäinen
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R F Pettersson
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P LehtovaaraL Kääriäinen
Feb 1, 2014·Science·Jennifer L HydeMichael S Diamond
Feb 16, 2015·Virology·Jennifer L Hyde, Michael S Diamond
Apr 3, 2015·Journal of Virology·K J SokoloskiR W Hardy
Apr 5, 2000·The Journal of Biological Chemistry·L VasiljevaL Kääriäinen
Feb 24, 2021·RNA Biology·Lukasz MarkiewiczPawel J Sikorski
Aug 1, 1981·Journal of Virology·D L SawickiL Kääriäinen

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